去卵巢小鼠学习记忆能力与海马超微结构的改变  

Changes of learning and memory function and hippocampus ultrastructure in ovariectomized mice

作　　者：魏明 田甜 刘鹏 甘露 Wei Ming;Tian Tian;Liu Peng;Gan Lu(Department of Pharmacology,the Xi'an Medical University,Xi'an 710021,China)

机构地区：[1]西安医学院基础医学部药理学教研室,陕西710021 [2]陕西省脑与神经疾病重点实验室 [3]西安医学院附属医院,陕西省人民医院妇科

出　　处：《脑与神经疾病杂志》2022年第4期246-248,共3页Journal of Brain and Nervous Diseases

基　　金：国家自然科学基金青年科学基金项目(81801414);陕西省教育厅2020年度重点科学研究计划项目(重点实验室项目)(20JS135);陕西省基础自然科学研究计划面上项目(2021JM-499)。

摘　　要：目的观察去卵巢(OVX)小鼠学习记忆能力及脑超微结构的改变。方法12只雌性C57BL/6小鼠随机分为假手术组(SHAM组),去卵巢组(OVX组),造模后继续喂养12w。采用Morris水迷宫测试认知能力,透射电镜检测小鼠大脑超微结构的改变。结果与SHAM组对比,OVX组小鼠的逃避潜伏期显著增加(P<0.05),穿越平台次数显著减少(P<0.05);OVX组小鼠海马神经元出现明显的超微结构改变,包括核膜破裂、核仁边界不清;线粒体数量减少、体积增大;高尔基体肿胀变大等。结论OVX小鼠的学习记忆能力下降,可能与海马超微结构的改变有关。Objective To study the effects of ovariectomized(OVX) on learning and memory ability and the changes of hippocampus ultrastructure in mice.Methods 12 female C57BL/6 mice were randomly divided into:SHAM,OVX group.Cognitive ability was detected by Morris water maze test after 12 weeks.Results Compared with Sham group,the evasive latency of OVX mice increased significantly(P=0.000<0.05),and the number of crossing platform decreased significantly(P=0.000<0.05),the results showed that there were obvious ultrastructural changes in the hippocampal neurons,including nuclear membrane rupture,unclear nucleolar boundary,decrease of the number and volume of Mitochondria,and enlargement of the Golgi apparatus.Conclusion The impairment of learning and memory in OVX mice may be relating to the ultrastructural changes of hippocampus.

关 键 词：去卵巢小鼠 海马 超微结构 

分 类 号：R741[医药卫生—神经病学与精神病学]