水稻紫色颖壳突变体的转录组分析  

Transcriptome Analysis of A Rice Glume Purple Mutant

作　　者：胡昌泉[1] 林觅 颜静宛[1] 林智敏[1] HU Chang-quan;LIN Mi;YAN Jing-wan;LIN Zhi-min(Institute of Biotechnology, FujianAcademy of Agricultural Sciences, Fuzhou, Fujian 350003, China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《福建农业科技》2022年第2期20-25,共6页Fujian Agricultural Science and Technology

基　　金：福建省自然科学基金项目(2018J01038)。

摘　　要：鉴定水稻花青素新基因及揭示其代谢机制,用于水稻新品种选育,是创制功能性水稻的手段之一。为探讨水稻紫色颖壳形成对花青素积累代谢途径的作用,以从水稻籼稻转化体组织培养中筛选获得的1个紫色颖壳杂合突变体(PiX)为材料,采用Illumina HiSeqTM 6000高通量转录组学(RNA-Seq)技术对其自交获得的突变型、正常型及野生型进行转录组测序研究。结果表明:突变型(ME)与野生型(F8)相比上调基因5108个、下调基因4288个,自交分离正常表型(PD)与野生型(F8)相比上调基因4653个、下调基因4397个,自交分离正常表型(PD)与突变型(ME)相比上调基因2078个、下调基因2829。GO功能富集分析表明水解酶活性类基因差异表达变化显著;KEGG Pathway分析结果表明,与野生型相比,在类黄酮生物合成途径中,突变型中与类黄酮合成相关色素基因数量多,且主要受编码查尔酮异构酶OsCHI影响。该研究结果可为研究水稻花色苷积累的分子机制提供参考。There is a way to create functional rice by identifying new rice anthocyanin genes and revealing their metabolic mechanism for the breeding of new rice varieties.In order to explore the effect of purple glume formation on anthocyanin accumulation and metabolism pathway in rice,a purple glume hybrid mutant(PiX)was screened from the tissue culture of Indica rice transformants,and the mutant,normal and wild-type transcripts obtained through its selfing was sequenced by Illumina hiseqtm 6000 high throughput transcriptome(RNA SEQ)technology.The results showed compared with wild-type(F8),mutant(ME)has 5108 up-regulated genes and 4288 down regulated genes,self segregation normal phenotype(PD)has 4653 up-regulated genes and 4397 down regulated genes,and self segregation normal phenotype(PD)has 2078 up-regulated genes and 2829 down regulated genes compared with mutant(ME).The Go function enrichment analysis showed that the differential expression of hydrolase activity genes changed significantly.The KEGG pathway analysis revealed that compared with the wild-type,the mutant had more pigment genes related to flavonoid synthesis in the flavonoid biosynthesis pathway,and those genes were mainly affected by the encoding chalcone isomerase OsCHI.The results can provide a reference for studying the molecular mechanism of anthocyanin accumulation in rice.

关 键 词：水稻 紫壳 花青素 转录组 

分 类 号：S511[农业科学—作物学]