结直肠癌患者肠道菌群组成及其代谢产物LPS和SCFAs的变化  

The changes of gut microbiota composition and metabolites LPS and SCFAs in patients with colorectal cancer

作　　者：李旭照[1] 王海[1] 白槟 马立旭[1] 赵建宁[2] 王宁萍[2] LI Xuzhao;WANG Hai;BAI Bing;MA Lixu;ZHAO Jianning;WANG Ningping(Department of Gerneral Surgery People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏回族自治区人民医院普外科,宁夏银川750002 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2022年第4期292-295,F0002,共5页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(2019AAC03169)。

摘　　要：目的研究结直肠癌患者肠道菌群及其代谢产物脂多糖(LPS)和短链脂肪酸(SCFAs)的变化规律。方法分别收集结直肠癌(CRC)患者和健康对照组的粪便标本,采用16S rRNA基因测序分析肠道菌群组成,采用鲎试剂法分析外周血LPS水平,采用气相色谱-质谱联用技术(GC-MS)检测肠道菌群代谢产物SCFAs含量的变化。结果与健康对照组相比,结直肠癌患者肠道菌群ɑ多样性发生显著变化,表明肠菌组成丰度明显改变。肠道菌群主坐标分析(PCoA)与非度量多维尺度分析(NMDS)结果显示,在健康对照组与结直肠癌患者之间差异有统计学意义,提示肠道菌群β多样性在该疾病状态下发生明显变化。进一步分析发现,结直肠癌患者肠道菌群在门水平上的厚壁菌门(Firmicutes)显著增加(P<0.05),而变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)显著降低(P<0.05);在属水平上显著增加的有肠杆菌(Enterobacteriaceae)、变形菌门(Prevotellaceae)、理研菌科(Rikenellaceae)、链球菌科(Streptococcaceae)、丹参科(Tannerellaceae)、脱硫弧菌科(Desulfovibrionaceae)、球菌科(Coriobacteriaceae)、巴内塞科(Barnesiellaceae),显著减少的菌有红球菌科(Ruminococcaceae)、拉氏菌科(Lachnospiraceae)。此外,肠道菌群来源的血浆LPS在结直肠癌中显著升高(P<0.05),而SCFAs包括乙酸、丙酸和丁酸却显著下降(P<0.05)。结论结直肠癌患者的肠道菌群组成及其代谢产物LPS和SCFAs有显著改变。Objective To analyze the changes of gut microbiota and its metabolites lipopolysaccharide(LPS)and short chain fatty acids(SCFAs)in patients with colorectal cancer.Methods Feces specimens were respectively isolated from patients with colorectal cancer(CRC)and healthy control(HC)and then detected by 16S rRNA gene sequencing.Tachypleus amebocyte lysate was used to measure the levels of LPS in plasma.Gas chromatography-mass spectrometry(GC-MS)was adopted to determine contents of SCFAs.Results Compared with healthy control,ɑ-diversity of gut microbiota showed significant altered in the patient group,demonstrating that the relative abundance of total microbial community was obviously changed in the patients with colorectal cancer.There were significant differences in principal coordinates analysis(PCoA)and non-metric multidimensional scaling(NMDS)of gut microbiota between the healthy control group and the patient group.Moreover,Firmicutes in the CRC group were significantly increased at the phylum level(P<0.05),but Proteobacteria and Bacteroidetes were decreased(P<0.05).Enterobacteriaceae,Prevotellaceae,Rikenellaceae,Streptococcaceae,Tannerellaceae,Desulfovibrionaceae,Coriobacteriaceae and Barnesiellaceae were elevated at the genus level,but Ruminococcaceae and Lachnospiraceae were reduced.In addition,the gut microbial LPS levels in plasma were increased(P<0.05),whereas SCFAs including acetic acid,propionic acid and butyric acid were down-regulated in the patients group(P<0.05).Conclusion The composition of gut microbiota and the metabolites SCFAs and LPS in the patients with colorectal cancer are significantly changed.

关 键 词：结直肠癌 肠道菌群 脂多糖 短链脂肪酸 

分 类 号：R735[医药卫生—肿瘤]