百里醌类似物ATQTHB对肺癌细胞株A549增殖与凋亡的影响  

Effects of thymoquinone analogue ATQTHB on proliferation and apoptosis of lung cancer A549 cells

作　　者：马刚[1] 纳莉[1] 王婷[2] 黄菱[2] 李凯璇 MA Gang;NA Li;WANG Ting;HUANG Ling;LI Kaixuan(Department of Respiratory and Gritical Care Medicine,General Hospital of Ningxia Medical University,Yinchuan 750004,China;School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院呼吸与危重症医学科,宁夏银川750004 [2]宁夏医科大学基础医学院,宁夏银川750004

出　　处：《宁夏医学杂志》2022年第4期302-304,共3页Ningxia Medical Journal

基　　金：宁夏医科大学校级科研课题(XY2017116);宁夏高等学校科学研究项目(NGY2017101)。

摘　　要：目的研究人工合成百里醌类似物ATQTHB对肺癌细胞A549的增殖抑制和诱导凋亡作用。方法将细胞分为5组:空白对照组、A549细胞组、A549细胞+ATQTHB干预(400μg/mL)组、人正常肺上皮细胞(BEAS-2B)组、BEAS-2B细胞+ATQTHB干预组。采用CCK-8法分析细胞增殖抑制情况,采用流式细胞术检测细胞凋亡水平,采用Western blot检测凋亡相关蛋白Bcl-2和Bax表达水平。结果与A549细胞组相比,400μg/mL ATQTHB干预对A549细胞增殖具有显著抑制作用(P<0.05);ATQTHB干预后,人正常肺上皮细胞BEAS-2B增殖与未干预细胞间差异无统计学意义(P>0.05);与A549细胞组相比,A549细胞+ATQTHB干预组的细胞凋亡率显著增加(P<0.05),抑制凋亡蛋白Bcl-2表达减少(P<0.05),促凋亡蛋白Bax表达增加(P<0.05);与BEAS-2B细胞组相比,BEAS-2B细胞+ATQTHB干预组的细胞凋亡率无显著改变(P>0.05),Bcl-2和Bax的蛋白表达水平差异无统计学意义(P>0.05)。结论研究证实了400μg/mL ATQTHB可能通过下调Bcl-2的表达、上调Bax的表达而促进肺癌细胞株A549的凋亡,发挥抑制肺癌细胞作用,具有潜在的应用价值。Objective To analyze the effects of artificially synthesized thymoquinone analogues ATQTHB on cell proliferation and apoptosis of lung cancer A549 cells.Methods The cells were divided into 5 groups:blank group,A549 group,A549+ATQTHB group,BEAS-2B group and BEAS-2B+ATQTHB group.CCK-8 was used to measure the cell proliferation and flow cytometry was used to detect cell apoptosis,western blot was used to determine the expression levels of apoptotic proteins including Bcl-2 and Bax.Results Compared to A549 group,cell apoptosis was dramatically increased in A549+ATQTHB group(P<0.05),whereas there was no significant difference between BEAS-2B group and BEAS-2B+ATQTHB group(P>0.05).Compared to A549 group,the expression level of Bcl-2 was decreased in A549+ATQTHB group(P<0.05)and the expression level of BAX was significantly increased(P<0.05).The expression level of Bcl-2 and BAX showed no significant difference between BEAS-2B group and BEAS-2B+ATQTHB group(P>0.05).Conclusion Thymoquinone analogues ATQTHB has the anti-tumor effects on lung cancer cells via suppressing cell proliferation by modulating the expression level of Bcl-2 and BAX.

关 键 词：百里醌类似物ATQTHB 肺癌 细胞增殖 凋亡 

分 类 号：R734[医药卫生—肿瘤]