冻干鼠疫F1抗体致敏血球的制备及其效价测定  

Preparation and titer determination of lyophilized plague F1 antibody sensitized blood cells

作　　者：张爱萍[1] 王梅[1] 于守鸿 赵小龙[1] 祁美英[1] 马龙 张鹏辉 刘中凯 李玲雯 史艳文 杨永海[1] ZHANG Ai-ping;WANG Mei;YU Shou-hong;ZHAO Xiao-long;QI Mei-ying;MA Long;ZHAG Peng-hui;LIU Zhong-kai;LI Ling-wen;SHI Yan-wen;YANG Yong-hai(Plague Prevention and Control Section,Qinghai Institute for Endemic Disease Prevention and Control,Xining 810021,China)

机构地区：[1]青海省地方病预防控制所鼠疫预防控制科,西宁810021

出　　处：《医学动物防制》2022年第3期306-306,F0003,F0004,共3页Journal of Medical Pest Control

基　　金：国家卫生健康委员会鼠疫防治研究重点实验室(共建)项目(2019PT310004);青海省鼠疫防控及研究重点实验室(2021-ZJ-Y15);青海省卫生健康系统适宜技术推广项目(2021-wjtg-04)。

摘　　要：目的筛选出适合野外鼠疫监测工作需求的冻干鼠疫F1抗体致敏血球。方法在传统鼠疫F1抗体致敏血球制备方法的基础上增加红细胞保护剂,制备冻干鼠疫F1抗体致敏血球,并通过血凝试验筛选出两种红细胞保护剂蔗糖与海藻糖及两种介质健康兔血清(normal rabbit serum, NRS)、小牛血清白蛋白(bovine serum albumin, BSA)与3种不同的样本稀释剂1%NRS、1%冻干NRS、1%冻干BSA的最佳配比关系。结果最佳的红细胞冻干保护剂为10%蔗糖和30 mmol/L海藻糖,最佳的介质为6%BSA,3种稀释剂中现配健康1%NRS的试验结果最好,1%冻干NRS需待试剂完全溶解方可进行试验,1%冻干BSA血球沉降差,结果不好判定。1%冻干NRS无需特殊保存,所以冻干鼠疫F1抗体致敏血球的最佳配比关系为30 mmol/L冻干海藻糖+6%BSA抗体致敏血球+1%冻干NRS与10%冻干蔗糖+6%BSA抗体致敏血球+1%冻干NRS,而且这两种血球的血凝效价均达到试验要求。结论新制备冻干鼠疫F1抗体致敏血球能满足鼠疫野外工作需求,无需特殊保存,携带方便,可在基层单位推广应用。Objective To screen out the freeze-dried plague F1 antibody sensitized blood cells suitable for field plague surveillance.Methods The erythrocyte protectant was added based on traditional preparation method of plague F1 antibody sensitized blood cells to prepare lyophilized plague F1 antibody sensitized blood cells.The optimal ratio between sucrose and trehalose, two kinds of erythrocyte protectants including sucrose and trehalose, two types of mediums including normal rabbit serum(NRS) and bovine serum albumin(BSA) and three types of different samples diluents with NRS in 1%,freeze-dried NRS in 1% and freeze-dried BSA in 1%.Results The optimal erythrocyte protectants were sucrose and trehalose with the concentration in 10% and 30 mmol/L respectively, and the optimal mediums was BSA with the concentration in 6%.In three diluents, the test results of healthy 1% NRS were the optimal.Freeze-dried NRS was allowed to be tested after the reagents were completely dissolved, and freeze-dried BSA was found to have poor blood cell sedimentation, causing the difficulty in determining the results.Freeze-dried 1% NRS required no special storage, the optimal ratio of freeze-dried plague F1 antibody sensitized blood cells was 30 mmol/L freeze-dried trehalose+6% BSA antibody sensitized blood cells+freeze-dried 1% NRS as well as 10% freeze-dried sucrose+6% BSA antibody sensitized blood cells+freeze-dried 1% NRS,and the hemagglutination titer of these two blood cells met the test requirements.Conclusion The freshly prepared freeze-dried plague F1 antibody sensitized blood cells can meet the requirements for plague field work, without special storage, are convenient to carry, and are supported to be popularized and applied in grass-roots organization.

关 键 词：冻干 鼠疫F1抗体致敏血球 应用评价 藏系绵羊红细胞 

分 类 号：R446.6[医药卫生—诊断学] R516.8[医药卫生—临床医学]