乳腺癌组织中HPV感染与TLR4、NF-κB蛋白表达的关系及其临床意义  

Relationship between HPV infection and expressions of TLR4,NF-κB protein in breast cancer and its clinical significance

作　　者：石喆[1] 任珊[2] 杨立[1] 戴文斌[1] SHI Zhe;REN Shan;YANG Li;DAI Wenbin(Department of Pathology,Liuzhou People’sHospital,Guangxi Zhuang Autonomous Region,Liuzhou545001,China;Department of Clinical Laboratory,Liuzhou People’s Hospital,Guangxi Zhuang Autonomous Region,Liuzhou545001,China)

机构地区：[1]广西壮族自治区柳州市人民医院病理科,广西柳州545001 [2]广西壮族自治区柳州市人民医院检验科,广西柳州545001

出　　处：《中国医药导报》2022年第8期11-15,共5页China Medical Herald

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20190114、Z2007306)。

摘　　要：目的探讨乳腺癌组织中人乳头瘤病毒(HPV)感染与Toll样受体4(TLR4)、核因子-κB(NF-κB)蛋白表达的关系及临床意义。方法选择广西壮族自治区柳州市人民医院乳腺外科2018年1月至2019年12月手术切除的浸润性癌乳腺标本50例为癌组、导管上皮非典型增生(ADH)乳腺标本30例为ADH组、正常乳腺组织30例为正常组。采用免疫组化染色方法检测三组HPV16/18E6、TLR4、NF-κB蛋白阳性表达情况,分析癌组及ADH组HPV16/18E6、TLR4、NF-κB蛋白之间的相关性,比较癌组HPV16/18E6、TLR4、NF-κB蛋白阳性表达与临床病理特征之间的关系。结果三组HPV16/18E6、TLR4与NF-κB蛋白阳性表达率比较,差异有高度统计学意义(P<0.01)。癌组HPV16/18E6阳性表达率高于ADH组和正常组(P<0.017);癌组TLR4、NF-κB蛋白阳性表达率高于ADH组、正常组,ADH组高于正常组(P<0.017)。癌组及ADH组中HPV16/18E6与TLR4、NF-κB蛋白呈正相关(P<0.01),TLR4与NF-κB蛋白呈正相关(P<0.01)。HPV16/18E6、TLR4及NF-κB蛋白阳性表达率与组织学分级、肿瘤直径、淋巴结转移及临床病理分期有关(P<0.05)。结论高危型HPV感染协同TLR4及NF-κB炎症相关因子在乳腺癌的发生、发展中起着重要的作用。Objective To investigate the relationship between human papilloma virus(HPV)infection and expressions of Toll-like receptor 4(TLR4)and nuclear factor-kappa B(NF-κB)protein in breast cancer and its clinical significance.Methods A total of 50 cases of invasive cancer breast samples from January 2018 to December 2019 in Department of Breast Surgery,Liuzhou People’s Hospital,Guangxi Zhuang Autonomous Region were classified as cancer group,30 cases of ductal atypical ductal hyperplasia(ADH)breast samples were classified as ADH group,and 30 cases of normal breast tissue were classified as normal group.Immunohistochemical staining was used to detect the positive expression of HPV16/18E6,TLR4,and NF-κB protein in three groups;the correlation between HPV16/18E6,TLR4,and NF-κB protein in cancer group and ADH group were analyzed;the relationship between positive expression of HPV16/18E6,TLR4,and NF-κB protein and clinicopathological features in cancer group were compared.Results The positive expression rate of HPV16/18E6,TLR4,and NF-κB protein among three groups were highly significantly different(P<0.01).The positive expression rate of HPV16/18E6 in cancer group was higher than that in ADH group and normal group(P<0.017);the positive expression rate of TLR4 and NF-κB protein cancer group was higher than that in ADH group and normal group,and ADH group was higher than normal group(P<0.017).In cancer group and ADH group,HPV16/18E6 were positively correlated with TLR4 and NF-κB protein(P<0.01),and TLR4 was positively correlated with NF-κB protein(P<0.01).The positive expression rate of HPV16/18E6,TLR4,and NF-κB protein were correlated with histological grade,tumor diameter,lymph node metastasis,and clinicopathological stage(P<0.05).Conclusion High-risk HPV infection plays an important role in the development and progression of breast cancer in conjunction with TLR4 and NF-κB inflammatory factors.

关 键 词：乳腺肿瘤 人乳头瘤病毒 TOLL样受体4 核因子-ΚB 

分 类 号：R737.9[医药卫生—肿瘤]