通窍益脑方对东莨菪碱痴呆模型小鼠学习记忆行为的影响  

Effect of Tongqiao Yinao Prescription on learning and memory behavior in Scopolamine dementia model mice

作　　者：阿力努尔·艾麦尔 聂雨楠 阿娜古丽·马合木提 高莉 Alinuer Aimaier;NIE Yunan;Anaer Mahemuti;GAO Li(College of Pharmacy,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urmuqi 830011,China;Xinjiang Uygur Autonomous Region Institute of Traditional Uighur MedicineXinjiang Key Laboratory of Uighur Medical Prescription,Xinjiang Uygur Autonomous Region,Urmuqi 830049,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]新疆维吾尔自治区维吾尔医药研究所新疆维吾尔医方剂学重点实验室,新疆乌鲁木齐830049

出　　处：《中国医药导报》2022年第10期30-33,共4页China Medical Herald

基　　金：新疆维吾尔自治区创新环境建设专项(天山雪松计划)(2019XS18);新疆维吾尔自治区公益性科研院所基金项目(KY2020052、KY2021057)。

摘　　要：目的探究通窍益脑方对东莨菪碱痴呆模型小鼠学习记忆行为的影响。方法50只雄性昆明小鼠采用随机数表法分为空白对照组、东莨菪碱模型组、多奈哌齐组、通窍益脑方0.41 g/kg组、通窍益脑方0.82 g/kg组,每组10只,连续灌胃给药29 d。除空白对照组外,其他各组行为学实验前30 min腹腔注射2 mg/kg氢溴酸东莨菪碱造模,空白对照组腹腔注射生理盐水。采用跳台试验检测小鼠学习记忆能力;采用比色法及酶联免疫吸附试验法检测各组海马组织中乙酰胆碱(ACh)、乙酰胆碱转移酶(ChAT)含量以及乙酰胆碱酯酶(AChE)活力。结果与空白对照组比较,在跳台试验的训练期和测试期,东莨菪碱模型组下台潜伏期缩短,下台错误次数增加,差异有统计学意义(P<0.05);与东莨菪碱模型组比较,在跳台试验的训练期和测试期,通窍益脑方0.41 g/kg组、通窍益脑方0.82 g/kg组下台潜伏期延长,下台错误次数减少,差异有统计学意义(P<0.05)。与空白对照组比较,东莨菪碱模型组ACh、ChAT含量下降,AChE活力升高,差异有统计学意义(P<0.05);与东莨菪碱模型组比较,通窍益脑方0.41 g/kg组、通窍益脑方0.82 g/kg组ACh、ChAT含量升高,AChE活力下降,差异有统计学意义(P<0.05)。通窍益脑方0.41 g/kg组与通窍益脑方0.82 g/kg组下台潜伏期,下台错误次数,ACh、ChAT含量,AChE活力比较,差异无统计学意义(P>0.05)。结论通窍益脑方可以在一定程度上改善东莨菪碱小鼠模型造成的短暂获得性学习记忆障碍,其作用机制与调节东莨菪碱小鼠模型的胆碱能功能相关。Objective To observe the effect of Tongqiao Yinao Prescription on learning and memory behavior in Scopolamine dementia model mice.Methods A total of 50 male Kunming mice were divided into blank control group,Scopolamine model group,Donepezil group,Tongqiao Yinao Prescription 0.41 g/kg group,and Tongqiao Yinao Prescription 0.82 g/kg group by random number table method,with ten mice in each group,continuously administrate drug for 29 d.Except blank control group,other groups were intraperitoneally injected with Scopolamine Hydrobromide 2 mg/kg 30 min before behavioral experiment to establish the model,blank control group was intraperitoneally injected with normal saline.The learning and memory ability of mice was tested by platform test,the contents of acetylcholine(ACh)and acetylcholinesterase(ChAT),and the activity of acetylcholinesterase(AChE)in hippocampus tissue of each group were determined by colorimetry and enzyme-linked immunosorbent assay.Results Compared with blank control group,the incubation period of stepping down in Scopolamine model group was shortened and the number of stepping down errors was increased during the training period and test period of platform test,and the differences were statistically significant(P<0.05);compared with Scopolamine model group,the incubation period of stepping down in Tongqiao Yinao Prescription 0.41 g/kg and Tongqiao Yinao prescription 0.82 g/kg group were prolonged and the number of stepping down errors were reduced during the training period and test period of platform test,and the differences were statistically significant(P<0.05).Compared with blank control group,the contents of ACh and ChAT of Scopolamine model group were decreased,and the activity of AChE was increased,and the differences were statistically significant(P<0.05);compared with Scopolamine model group,the contents of ACh and ChAT of Tongqiao Yinao Prescription 0.41 g/kg and Tongqiao Yinao prescription 0.82 g/kg group were increased,and the activity of AChE were decreased,and the differences were st

关 键 词：通窍益脑方 东莨菪碱模型 学习记忆 乙酰胆碱 

分 类 号：R749.16[医药卫生—神经病学与精神病学]