miR-21在恩度联合X线照射心肌纤维母细胞的作用  

The role of miR-21 in Endostar combined with X-ray irradiation of cardiac fibroblasts

作　　者：曹东东 欧阳伟炜 赵星[3] 苏胜发[1] 章俊 付士美 冯张鑫 李青松 马筑[1] 杨文刚 陈霞霞 卢冰 Cao Dongdong;Ouyang Weiwei;Zhao Xing;Su Shengfa;Zhang Jun;Fu Shimei;Feng Zhangxin;Li Qingsong;Ma Zhu;Yang Wengang;Chen Xiaxia;Lu Bing(Affiliated Hospital of Guizhou Medical University/Department of Oncology,Affiliated Cancer Hospital of Guizhou Medical University,Guiyang 550004,China;Teaching and Research Section of Oncology,Guizhou Medical University,Guiyang 550004,China;Stem Cell and Tissue Engineering Research Center,Guizhou Medical University,Guiyang 550004,China;Department of Pathology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学附属医院/贵州医科大学附属肿瘤医院肿瘤科,贵阳550004 [2]贵州医科大学肿瘤学教研室,贵阳550004 [3]贵州医科大学组织工程与干细胞实验中心,贵阳550004 [4]贵州医科大学附属医院病理科,贵阳550004

出　　处：《中华放射肿瘤学杂志》2022年第4期370-375,共6页Chinese Journal of Radiation Oncology

基　　金：国家自然科学基金(NSFC81660507);中国国际医学交流基金会(Z-2014-06-19393);贵州省科技厅科技合作项目([2014]7135);贵州省应用基础研究计划重大项目([2015]2003)。

摘　　要：目的初步研究恩度联合X线照射对心肌纤维母细胞(CF)影响及miR-21的作用。方法使用大鼠CF进行实验,设空白对照组、10Gy X线照射组、恩度组、10Gy X线+恩度组、10Gy X线+恩度+NC mimics组(阴性对照组1)、10Gy X线+恩度+miR-21 mimics组、10Gy X线+恩度+NC抑制剂组(阴性对照组2)、10Gy X线+恩度+miR-21抑制剂组。MTT法检测CF增殖,蛋白印迹法检测胶原蛋白I(CollagenⅠ)的表达、q-PCR检测CollagenⅠ和miR-21 mRNA的表达。结果与空白对照组、10Gy X线+恩度组及阴性对照组1比较,10Gy X线+恩度+miR-21 mimics组CF增殖及CollagenⅠmRNA、miR-21 mRNA表达增加(均P<0.05)。与空白对照组、10Gy X线+恩度组及阴性对照组2比较,10Gy X线+恩度+miR-21抑制剂组的CF增殖及CollagenⅠmRNA表达降低(均P<0.05)。结论模拟miR-21基因表达CF增殖和CollagenⅠ表达增加,抑制该基因表达CF增殖和CollagenⅠ表达降低。Objective To investigate the implication of micro RNA-21(miR-21)in Endostar combined with X-ray irradiation of cardiac fibroblasts(CF).Methods Rat CFs were used in this experiment and been divided into the blank control group,10Gy X-ray irradiation group,Endostar group,10Gy X-ray+Endostar group,10Gy X-ray+Endostar+NC mimic group(negative control 1),10Gy X-ray+Endostar+miR-21 mimic group,10Gy X-ray+Endostar+NC inhibitor group(negative control 2)and 10Gy X-ray+Endostar+miR-21 inhibitor group.The proliferation of CF was determined by Methyl thiazolyl tetrazolium(MTT)assay.The expression level of CollagenⅠprotein was analyzed by Western blot.The expression levels of CollagenⅠand miR-21 mRNA were assayed by real-time quantitative polymerase chain reaction(q-PCR).Results In the 10Gy X-ray+Endostar+miR-21 mimic group,the CF proliferation,CollagenⅠand miR-21 mRNA were increased significantly compared with those in the blank control group,10Gy X-ray+Endostar group,and negative control group 1(all P<0.05).In the 10Gy X-ray+Endostar+miR-21 inhibitor group,the CF proliferation and expression levels of CollagenⅠmRNA were decreased significantly compared with those in the blank control group,10Gy X-ray+Endostar group and negative control group 2(all P<0.05).Conclusions The CF proliferation and CollagenⅠexpression are increased when the expression level of miR-21 gene is simulated.When inhibiting the expression of miR-21 gene,the CF proliferation and CollagenⅠexpression are reduced.

关 键 词：miR-21基因 恩度 心肌纤维母细胞 X线照射 

分 类 号：R730.5[医药卫生—肿瘤] R542.23[医药卫生—临床医学]