Micropatterns and peptide gradient on the inner surface of a guidance conduit synergistically promotes nerve regeneration in vivo  

作　　者：Deteng Zhang Ziming Li Haifei Shi Yuejun Yao Wang Du Pan Lu Kejiong Liang Liangjie Hong Changyou Gao 

机构地区：[1]MOE Key Laboratory of Macromolecular Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou,310027,China [2]Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine,Zhejiang University,Hangzhou,310058,China [3]Department of Hand Surgery,First Affiliated Hospital of Zhejiang University,School of Medicine.Hangzhou,310009,China

出　　处：《Bioactive Materials》2022年第3期134-146,共13页生物活性材料（英文）

基　　金：This study is financially supported by the National Key Research and Development Program of China(2016YFC1100403);the National Natural Science Foundation of China(21434006,51873188);and the Fundamental Research Funds for the Central Universities of China(2020XZZX004-01).

摘　　要：Both of the surface topographical features and distribution of biochemical cues can influence the cell-substrate interactions and thereby tissue regeneration in vivo.However,they have not been combined simultaneously onto a biodegradable scaffold to demonstrate the synergistic role so far.In this study,a proof-of-concept study is performed to prepare micropatterns and peptide gradient on the inner wall of a poly(D,L-lactide-co-caprolactone)(PLCL)guidance conduit and its advantages in regeneration of peripheral nerve in vivo.After linear ridges/grooves of 20/40μm in width are created on the PLCL film,its surface is aminolyzed in a kinetically controlled manner to obtain the continuous gradient of amino groups,which are then transferred to CQAASIKVAV peptide density gradient via covalent coupling of glutaraldehyde.The Schwann cells are better aligned along with the stripes,and show a faster migration rate toward the region of higher peptide density.Implantation of the nerve guidance conduit made of the PLCL film having both the micropatterns and peptide gradient can significantly accelerate the regeneration of sciatic nerve in terms of rate,function recovery and microstructures,and reduction of fibrosis in muscle tissues.Moreover,this nerve conduit can also benefit the M2 polarization of macrophages and promote vascularization in vivo.

关 键 词：Peptides gradient MICROPATTERNS Contact guidance effect Nerve guidance conduits Nerve regeneration 

分 类 号：R745[医药卫生—神经病学与精神病学] R318.08[医药卫生—临床医学]