TRIP13在非小细胞肺腺癌中促增殖侵袭和血管生成的影响及作用机制  

Effect and Mechanism of Action of TRIP13 on Proliferation Invasion and Angiogenesis in NSCLC

作　　者：董岩[1] 李佳蔚 贾依娜尔[1] 黄咏梅[1] 张峤[1] DONG Yan;LI Jiawei;JIA Yinaer(The Affiliated Tumor Hospital of Xinjiang Medical University, Xinjiang Urumqi 830000, China)

机构地区：[1]新疆医科大学附属肿瘤医院,新疆乌鲁木齐830000

出　　处：《河北医学》2022年第4期534-539,共6页Hebei Medicine

基　　金：新疆维吾尔自治区卫生健康青年医学科技人才专项,(编号:WJWY-201907)。

摘　　要：目的:研究甲状腺激素受体作用因子13(Thyroid hormone receptor interactor 13,TRIP13)对非小细胞肺癌(Non-small-cell lung cancer,NSCLC)增殖、侵袭和血管生成的影响及作用机制。方法:收集45例NSCLC患者肿瘤及其对应癌旁组织,通过qRT-PCR检测肿瘤和癌旁组织中TRIP13表达量并分析其与患者临床特征的相关性。检测人肺腺癌细胞系A549和肺鳞癌细胞系SKMES1 TRIP13表达量。通过慢病毒转染法将PLK-TRIP13转染至A549和SKMES1,分析其与NSCLC细胞增殖、侵袭和血管生成的关系。结果:与癌旁组织相比,TRIP13在肿瘤组织高表达。TRIP13的高表达与肿瘤类型、肿瘤分期和淋巴结转移相关。过表达TRIP13显著促进A549细胞增殖、侵袭和血管生成,而对SKMES1细胞增殖、侵袭和血管生成的促进作用不显著。结论:TRIP13能够促进非小细胞肺腺癌的增殖、侵袭和血管生成,检测TRIP13与NSCLC病理类型可以作为一种潜在的肺腺癌临床治疗方案。Objective:To investigate the effects of thyroid hormone receptor interactor 13(TRIP13)on proliferation,invasion and angiogenesis in non-small-cell lung cancer(NSCLC)and its related mechanisms.Methods:Forty-five NSCLC patients'tumours and their corresponding paraneoplastic tissues were collected.The expression of TRIP13 in the tumours and paraneoplastic tissues was measured by qRT-PCR and analysed for correlation with the patients'clinical characteristics.The expression of TRIP13 in human lung adenocarcinoma cell line A549 and lung squamous carcinoma cell line SKMES1 was measured.PLK-TRIP13 was transfected into A549 and SKMES1 by lentiviral transfection,and its relationship with NSCLC cell proliferation,invasion and angiogenesis was analysed.Results:TRIP13 was highly expressed in tumor tissues compared with adjacent tissues.TRIP13 high expression correlated with tumor type,tumor stage,and lymph node metastasis.TRIP13 high expression significantly promoted proliferation,invasion and angiogenesis of A549 cells,but not of SKMES1 cells.Conclusion:TRIP13 can promote proliferation,invasion and angiogenesis of non-small cell lung adenocarcinoma,and detection of TRIP13 and NSCLC pathological types can serve as a potential clinical treatment option for lung adenocarcinoma.

关 键 词：非小细胞肺癌 腺癌 甲状腺激素受体作用因子13 增殖和侵袭 血管生成 

分 类 号：R73[医药卫生—肿瘤]