自发性脑出血患者血清microRNA-130a、microRNA-210表达水平及与早期神经功能恶化的关系  

The correlation between serum levels of microRNA-130a and microRNA-210 and early neurological deterioration in patients with spontaneous intracerebral hemorrhage

作　　者：李光波 王新港 张旭伟[1] Guang-bo Li;Xin-gang Wang;Xu-wei Zhang(Department of Neurosurgery,Lianyungang Hospital of Traditional Chinese Medicine,Lianyungang,Jiangsu 221000,China)

机构地区：[1]连云港市中医院神经外科,江苏连云港221000

出　　处：《中国现代医学杂志》2022年第8期79-84,共6页China Journal of Modern Medicine

基　　金：2020年江苏省重点研发计划(No:BE2020617)。

摘　　要：目的探讨自发性脑出血(ICH)患者血清microRNA-130a(miR-130a)、microRNA-210(miR-210)表达水平及与早期神经功能恶化(END)的关系。方法选取2018年7月—2021年6月连云港市中医院收治的ICH患者82例作为ICH组,另取同期该院60例健康体检者作为对照组。采用逆转录聚合酶链反应检测研究对象血清miR-130a、miR-210。依据ICH患者是否发生END分为END组和非END组,分析其临床资料。采用一般多因素Logistic回归模型分析ICH患者发生END的因素;绘制受试者工作特征(ROC)曲线,分析血清miR-130a、miR-210对ICH患者发生END的预测效能。结果ICH组血清miR-130a、miR-210高于对照组(P<0.05)。ICH患者END发生率为20.73%。END组入院收缩压(SBP)、入院格拉斯哥昏迷(GCS)评分、入院美国国立卫生研究院卒中量表(NIHSS)评分、血肿破入脑室占比、miR-130a、miR-210均高于非END组(P<0.05),入院血肿体积大于非END组(P<0.05)。一般多因素Logistic回归分析结果显示,血肿体积[O^R=2.846(95%CI:1.253,6.784)]、血肿破入脑室[O^R=2.787(95%CI:1.877,5.862)]、miR-130a[O^R=3.347(95%CI:2.475,8.275)]及miR-210[O^R=3.086(95%CI:2.051,7.436)]是ICH患者发生END的影响因素(P<0.05)。ROC曲线结果显示,血清miR-130a、miR-210预测ICH患者发生END的曲线下面积分别为0.824(95%CI:0.724,0.899)、0.868(95%CI:0.776,0.933)。结论血清miR-130a、miR-210在ICH患者中高表达,可影响END的发生,并作为评估ICH患者发生END的重要参考指标。Objective To investigate the correlation between the serum levels of microRNA-130a(miR-130a)and microRNA-210(miR-210)and early neurological deterioration(END)in patients with spontaneous intracerebral hemorrhage(ICH).Methods A total of 82 ICH patients admitted to our hospital from July 2018 to June 2021 were selected as the ICH group,and 60 healthy volunteers who underwent physical examinations in our hospital during the same time period were selected as the control group.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect serum miR-130a and miR-210 levels in all subjects.According to whether ICH patients developed END,they were divided into END group and non-END group and the clinical data of patients in the two groups were compared.Logistic regression analysis was performed to determine the factors affecting the occurrence of END in ICH patients.The receiver operating characteristic(ROC)curve was plotted,and the area under the ROC curve(AUC)was used to analyze the predictive values of serum miR-130a and miR-210 for the occurrence of END in ICH patients.Results The serum levels of miR-130a and miR-210 in the ICH group were higher than those in the control group(P<0.05).The incidence of END in ICH patients in our study was 20.73%.In the END group,admission systolic blood pressure(SBP),admission Glasgow Coma Scale(GCS)score,admission National Institutes of Health Stroke Scale(NIHSS)score,hematoma volume,percentage of hematoma with blood extension into the ventricles,and serum levels of miR-130a and miR-210 were higher than those in the non-END group(P<0.05).Logistic regression analysis showed that the volume of hematoma[O^R=2.846(95%CI:1.253,6.784)],the hematoma with blood extension into the ventricles[O^R=2.787(95%CI:1.877,5.862)],serum miR 130a[O^R=3.347(95%CI:2.475,8.275)]and serum miR-210[O^R=3.086(95%CI:2.051,7.436)]were factors affecting the occurrence of END in ICH patients(P<0.05).ROC analysis revealed that the AUC of serum miR-130a and miR-210 for predicting the occurrence of END in

关 键 词：自发性脑出血 microRNA-130a microRNA-210 早期神经功能恶化 

分 类 号：R743.3[医药卫生—神经病学与精神病学]