鸡蛋壳负载的纳米氧化锌对大肠杆菌的体外抑菌作用  

In vitro antibacterial effect of eggshell-loaded nano-zinc oxide on Escherichia coli

作　　者：陈雅瑜[1] 许婉卿[1] 陈芳川 戴秋婷 丁志雄[1] 黄聪明[1] 郑丹萍 赖顺霞[1] CHEN Yayu;XU Wanqing;CHEN Fangchuan;DAI Qiuting;DING Zhixiong;HUANG Congming;ZHENG Danping;LAI Shunxia(Department of Stomatology,the Second Affiliated Hospital of Fujian Medical University,Quanzhou362000,China)

机构地区：[1]福建医科大学附属第二医院口腔科,福建泉州362000

出　　处：《中国现代医生》2022年第8期16-19,F0003,共5页China Modern Doctor

基　　金：福建省泉州市科技计划项目(2018Z124)。

摘　　要：目的牙科设备水管经常被微生物膜污染,微生物膜中含大量有害微生物,大肠杆菌是其主要的微生物之一。蛋壳作为日常生活中的废弃物,将其废物利用负载纳米氧化锌。为寻找牙科设备水管的新型消毒剂,探讨研究蛋壳负载纳米氧化锌对大肠杆菌的影响。方法鸡蛋壳溶解硝酸钾溶液中,经过过滤煅烧溶解冷却,得到鸡蛋壳负载的纳米氧化锌,电镜观察蛋壳及蛋壳负载的纳米氧化锌形态。选用大肠杆菌ATCC 25922作为实验菌株。测定蛋壳负载纳米氧化锌对大肠杆菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果纳米氧化锌对大肠杆菌的最低抑菌浓度640μg/ml,最低杀菌浓度为5120μg/ml。结论蛋壳负载的纳米氧化锌能抑制大肠杆菌的生长,可以作为牙科设备水管的新型消毒剂。Objective The water pipe of dental equipment is often polluted by microbial membrane,which contains a large number of harmful microorganisms,and Escherichia coli is one of the main microorganisms.Eggshell as a waste in daily life,its waste is used to load nano-zinc oxide.The effect of eggshell-loaded nano-zinc oxide on Escherichia coli was studied in order to find a new disinfectant for the dental equipment water pipe.Methods The eggshell was dissolved in potassium nitrate solution,and eggshell-loaded nano-zinc oxide was obtained after filtration,calcination,dissolved and cooling.The morphology of eggshell and eggshell-loaded nano-zinc oxide was observed under the electron microscope.Escherichia coli ATCC 25922 was selected as the experimental strain.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of eggshell-loaded nano-zinc oxide on Escherichia coli were determined.Results The minimum inhibitory concentration and bactericidal concentration of nano-zinc oxide on Escherichia coli were 640μg/ml and 5120μg/ml respectively.Conclusion Eggshell-loaded nano-zinc oxide inhibits the growth of Escherichia coli and can be used as a new disinfectant for dental equipment water pipes.

关 键 词：鸡蛋壳 纳米氧化锌 大肠杆菌 抑菌 

分 类 号：R378.2[医药卫生—病原生物学]