iPSC-derived cranial neural crest-like cells can replicate dental pulp tissue with the aid of angiogenic hydrogel  

作　　者：Yoshifumi Kobayashi Julie Nouet Erdenechimeg Baljinnyam Zain Siddiqui Daniel HFine Diego Fraidenraich Vivek A.Kumar Emi Shimizu 

机构地区：[1]Department of Oral Biology,Rutgers School of Dental Medicine,Newark,NJ,07103,USA [2]Department of Cell Biology and Molecular Medicine,Rutgers New Jersey Medical School,Newark,NJ,07103,USA [3]Department of Biomedical Engineering,New Jersey Institute of Technology,Newark,NJ,07102,USA

出　　处：《Bioactive Materials》2022年第8期290-301,共12页生物活性材料（英文）

基　　金：supported by NIH grants,R01DE025885(E.S),R15EY029504(VAK);National Science Foundation NSF IIP 1903617(VAK).

摘　　要：The dental pulp has irreplaceable roles in maintaining healthy teeth and its regeneration is a primary aim of regenerative endodontics.This study aimed to replicate the characteristics of dental pulp tissue by using cranial neural crest(CNC)-like cells(CNCLCs);these cells were generated by modifying several steps of a previously established method for deriving NC-like cells from induced pluripotent stem cells(iPSCs).CNC is the anterior region of the neural crest in vertebrate embryos,which contains the primordium of dental pulp cells or odontoblasts.The produced CNCLCs showed approximately 2.5-12,000-fold upregulations of major CNC marker genes.Furthermore,the CNCLCs exhibited remarkable odontoblastic differentiation ability,especially when treated with a combination of the fibroblast growth factors(FGFs)FGF4 and FGF9.The FGFs induced odontoblast marker genes by 1.7-5.0-fold,as compared to bone morphogenetic protein 4(BMP4)treatment.In a mouse subcutaneous implant model,the CNCLCs briefly fated with FGF4+FGF9 replicated dental pulp tissue characteristics,such as harboring odontoblast-like cells,a dentin-like layer,and vast neovascularization,induced by the angiogenic self-assembling peptide hydrogel(SAPH),SLan.SLan acts as a versatile biocompatible scaffold in the canal space.This study demonstrated a successful collaboration between regenerative medicine and SAPH technology.

关 键 词：Regenerative endodontics IPSC Cranial neural crest Fibroblast growth factor Odontoblastic differentiation Angiogenic self-assembling peptide hydrogel 

分 类 号：R78[医药卫生—口腔医学]