新疆荒漠肉苁蓉粗多糖配伍流感病毒疫苗的抗原节约作用  

Antigen sparing with influenza virus vaccine formulated with crude polysaccharides from Cistanche deserticola Y.C.Ma adjuvant

作　　者：肖鹏 赵兵[1] 王丹阳[1] 张爱莲[1] Xiao Peng;Zhao Bing;Wang Danyang;Zhang Ailian(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源与基因工程重点实验室,乌鲁木齐830046

出　　处：《中华微生物学和免疫学杂志》2022年第3期216-223,共8页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(31960164,31660259)。

摘　　要：目的:探究新疆荒漠肉苁蓉粗多糖(crude polysaccharides from Cistanche deserticola Y.C.Ma, CPCD)配伍流感病毒疫苗(influenza virus vaccine,IVV)的抗原节约作用。 方法:一定剂量的CPCD配伍低剂量(0.01 μg)或高剂量(0.1 μg)的IVV皮下途径免疫小鼠,血凝抑制(hemagglutinin inhibition, HI)试验检测血清中HI抗体滴度;间接ELISA法检测血清中特异性抗体及亚型的水平;MTT法检测脾脏淋巴细胞增殖水平;流式细胞术检测脾脏细胞中T淋巴细胞亚群及胞内细胞因子IFN-γ的表达水平。结果:CPCD可以显著增强血清中HI抗体滴度(234.67±47.70 vs 149.33±47.70, P<0.05),促进特异性IgG( A450值:1.16±0.63 vs 0.30±0.21, P<0.05)和IgG1( A450值:1.09±0.60 vs 0.26±0.21, P<0.05)抗体水平的提高,并促进脾脏淋巴细胞增殖( P<0.05)。CPCD还可显著提高CD4 +[(41.97±4.58)% vs (25.43±1.48)%, P<0.05]、CD8 +[(12.67±0.33)% vs (9.02±1.07)%, P<0.05]及CD4 +CD44 +[(11.77±0.69)% vs (8.64±0.71)%, P<0.05]、CD8 +CD44 +[(6.70±0.67)% vs (4.66±0.39)%, P<0.05] T淋巴细胞亚群的比例及胞内细胞因子IFN-γ[CD4 +:(1.36±0.07)% vs (0.87±0.06)%, CD8 +:(2.09±0.20)% vs (1.42±0.08)%, P均<0.05]的分泌水平。CPCD配伍IVV的低剂量组和IVV高剂量组之间差异无统计学意义( P>0.05),显示可以节约10倍IVV抗原用量。 结论:CPCD配伍IVV可以显著增强体液免疫应答和细胞免疫应答,具有抗原节约作用,为IVV新型佐剂的研究提供实验参考,并具有应用于季节性流感和流感大流行防治的潜力。Objective To investigate the antigen-sparing effects of crude polysaccharides from Cistanche deserticola Y.C.Ma(CPCD)for influenza virus vaccine(IVV).Methods ICR mice were immunized subcutaneously with CPCD combined with different doses of IVV(0.01μg and 0.1μg).Hemagglutinin inhibition(HI)assay was used to detect HI titers in serum samples.Indirect ELISA was performed to detect the levels of specific IgG antibodies and their subtypes in serum samples.The proliferation of splenic lymphocytes was detected by MTT assay.The percentages of CD4+,CD8+and CD44+T cells and the levels of IFN-γin splenic cells isolated from the vaccinated mice were analyzed by flow cytometry.Results CPCD significantly increased HI titers(234.67±47.70 vs 149.33±47.70,P<0.05),promoted the production of IgG(A450 value:1.16±0.63 vs 0.30±0.21,P<0.05)and IgG1(A450 value:1.09±0.60 vs 0.26±0.21,P<0.05)and enhanced splenic lymphocyte proliferation(P<0.05).CPCD also significantly up-regulated the expression of CD4+[(41.97±4.58)%vs(25.43±1.48)%,P<0.05],CD8+[(12.67±0.33)%vs(9.02±1.07)%,P<0.05],CD4+CD44+[(11.77±0.69)%vs(8.64±0.71)%,P<0.05]and CD8+CD44+[(6.70±0.67)%vs(4.66±0.39)%,P<0.05]T cell subsets as well as the secretion of IFN-γin CD4+[(1.36±0.07)%vs(0.87±0.06)%,P<0.05]and CD8+[(2.09±0.20)%vs(1.42±0.08)%,P<0.05]T cells.In addition,there was no significant difference between CPCD combined with low-dose IVV group and high-dose IVV alone group(P>0.05),implying a 10-fold antigen sparing.Conclusions CPCD,as an adjuvant for influenza virus vaccine,could enhance humoral and cellular immune responses and reduce antigen dose,which might be a potential adjuvant for seasonal or pandemic influenza vaccines.

关 键 词：流感病毒疫苗 肉苁蓉 粗多糖 抗原节约 

分 类 号：R392[医药卫生—免疫学]