Optimal regenerative repair of large segmental bone defect in a goat model with osteoinductive calcium phosphate bioceramic implants  

作　　者：Wei Zhi Xiaohua Wang Dong Sun Taijun Chen Bo Yuan Xiangfeng Li Xuening Chen Jianxin Wang Zhao Xie Xiangdong Zhu Kai Zhang Xingdong Zhang 

机构地区：[1]Key Laboratory of Advanced Technologies of Materials,Ministry of Education,Southwest Jiaotong University,Chengdu,610031,China [2]Department of Orthopaedics,First Affiliated Hospital,Third Military Medical University(Army Medical University),Gaotanyan No.30,400038,Chongqing,China [3]National Engineering Research Center for Biomaterials,College of Biomedical Engineering,Sichuan University,Chengdu,610064,China

出　　处：《Bioactive Materials》2022年第5期240-253,共14页生物活性材料（英文）

基　　金：supported by the National Key R&D Program of China(2016YFC1102000);Research on repair technology and equipment of war injury(AWS17J004-02);the Science and Technology Innovation Seedling Project of Sichuan Province,China(2021057).

摘　　要：So far,how to achieve the optimal regenerative repair of large load-bearing bone defects using artificial bone grafts is a huge challenge in clinic.In this study,a strategy of combining osteoinductive biphasic calcium phosphate(BCP)bioceramic scaffolds with intramedullary nail fixation for creating stable osteogenic microenvironment was applied to repair large segmental bone defects(3.0 cm in length)in goat femur model.The material characterization results showed that the BCP scaffold had the initial compressive strength of over 2.0 MPa,and total porosity of 84%.The cell culture experiments demonstrated that the scaffold had the excellent ability to promote the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells(BMSCs).The in vivo results showed that the intramedullary nail fixation maintained the initial stability and structural integrity of the implants at early stage,promoting the osteogenic process both guided and induced by the BCP scaffolds.At 9 months postoperatively,good integration between the implants and host bone was observed,and a large amount of newborn bones formed,accompanying with the degradation of the material.At 18 months postoperatively,almost the complete new bone substitution in the defect area was achieved.The maximum bending strength of the repaired bone defects reached to the 100% of normal femur at 18 months post-surgery.Our results demonstrated the good potential of osteoinductive BCP bioceramics in the regenerative repair of large load-bearing bone defects.The current study could provide an effective method to treat the clinical large segmental bone defects.

关 键 词：Calcium phosphate bioceramics Large segmental bone defects Bone regenerative repair OSTEOINDUCTION Mechanical stability 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]