Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect  

作　　者：Haoran Wang Honghui Liu Yunfei Guo Wenjing Zai Xianghui Li Wei Xiong Xiaozhi Zhao Yingfang Yao Yiqiao Hu Zhigang Zou Jinhui Wu 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,Chemistry and Biomedicine Innovation Center,Medical School and School of Life Sciences,Nanjing University,Nanjing,210093,China [2]National Laboratory of Solid State Microstructures,Collaborative Innovation Center of Advanced Microstructures,School of Physics,Nanjing University,Nanjing,210093,China [3]School of Basic Medical Science,Nanjing University of Chinese Medicine,Nanjing,China [4]Jiangsu Key Laboratory for Nano Technology,Nanjing University,Nanjing,210093,China [5]Department of Urology,Drum Tower Hospital,Medical School,Nanjing University,Nanjing,210093,China

出　　处：《Bioactive Materials》2022年第6期97-106,共10页生物活性材料（英文）

基　　金：supported by National Key R&D Program of China(2017YFA0205400);National Natural Science Foundation of China(No.31872755,81872811,32171372);Jiangsu Outstanding Youth Funding(BK20190007);supported by the Central Fundamental Research Funds for the Central Universities(02141438473).

摘　　要：The ideal photodynamic therapy(PDT)should effectively remove the primary tumor,and produce a stronger immune memory effect to inhibit the tumor recurrence and tumor metastasis.However,limited by the hypoxic and immunosuppressive microenvironment,the PDT efficiency is apparently low.Here,Chlorella(Chl.)is exploited to enhance local effect by producing oxygen to reverse hypoxia,and release adjuvants to reverse immunosuppressive microenvironment to enhance abscopal effect afterwards.Results from different animal models indicated that Chl.could enhance local effect and PDT related immune response.Ultimately,Chl.coupled PDT elicited anti-tumor effects toward established primary tumors(inhibition rate:90%)and abscopal tumors(75%),controlled the challenged tumors(100%)and alleviated metastatic tumors(90%).This Chl.coupled PDT strategy can also produce a stronger anti-tumor immune memory effect.Overall,this Chl.coupled PDT strategy generates enhanced local tumor killing,boosts PDT-induced immune responses and promotes anti-tumor immune memory effect,which may be a great progress for realizing systemic effect of PDT.

关 键 词：CHLORELLA Photodynamic therapy Controlled adjuvants release Systemic antitumor immune response Immune memory effect 

分 类 号：R73[医药卫生—肿瘤] R454.2[医药卫生—临床医学]