Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression  

作　　者：Junjun Yang Xin Wang Yahan Fan Xiongbo Song Jiangyi Wu Zhenlan Fu Tao Li Yang Huang ZheXiong Tang Shuo Meng Na Liu Jiajia Chen Pingju Liu Liu Yang Xiaoyuan Gong Cheng Chen 

机构地区：[1]Center for Joint Surgery,Southwest Hospital,Army Medical University(Third Military Medical University),Chongqing,400038,China [2]College of Medical Informatics,Chongqing Medical University,Chongqing,400016,China [3]Blood Transfusion Department,Southwest Hospital,Army Medical University(Third Military Medical University),Chongqing,400038,China [4]Department of Sports Medicine,Peking University Shenzhen Hospital,Peking University,Shenzhen,518036,China [5]Key Laboratory of Freshwater Fish Reproduction and Development,Ministry of Education,Laboratory of Molecular Developmental Biology,School of Life Sciences,Southwest University,Chongqing,400038,China [6]Biomedical Analysis Center,Army Medical University(Third Military Medical University),Chongqing,400038,China [7]Department of Orthopedics,Zunyi Traditional Chinese Medicine Hospital,Zunyi,563099,China

出　　处：《Bioactive Materials》2022年第4期443-459,共17页生物活性材料（英文）

基　　金：supported by CHONGQING TALENTS PROJECT(4246ZJ1);Science and technology projects of Chongqing Education Commission(KJQN202000427).

摘　　要：Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in the joint cavity.A recent study shows that tropoelastin(TE)regulates adhesion,proliferation and phenotypic maintenance of MSCs as a soluble additive,indicating that TE could promote MSCs-homing in regenerative medicine.In this study,we used TE as injection medium,and compared it with classic media in MSCs intra-articular injection such as normal saline(NS),hyaluronic acid(HA),and platelet-rich plasma(PRP).We found that TE could effectively improve adhesion,migration,chondrogenic differentiation of infrapatellar fat pad MSCs(IPFP-MSCs)and enhance matrix synthesis of osteoarthritic chondrocytes(OACs)in indirect-coculture system.Moreover,TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1,ERK1/2 and vinculin(VCL)in vitro.In addition,intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues.Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo.In conclusion,TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway.Our findings may provide new insights in MSCs intra-articular injection for OA treatment.

关 键 词：TROPOELASTIN OSTEOARTHRITIS Infrapatellar fat pad MSCs 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]