lncRNA SCAMP1靶向miR-197-3p对肝癌SNU-449细胞增殖和侵袭的影响  

Effect of lncRNA SCAMP1 targeting miR-197-3p on the proliferation and invasion of hepatocellular carcinoma SNU-449 cells

作　　者：杜全顺 李少华[1] 马成云 贾振飞[1] 王现兵[1] 王嘉毅[1] DU Quan-shun;LI Shao-hua;MA Cheng-yun;JIA Zhen-fei;WANG Xian-bing;WANG Jia-yi(Department of General Surgery,Anyang People's Hospital,Anyang 455100,China)

机构地区：[1]安阳市人民医院普外科,河南安阳455100

出　　处：《中国现代普通外科进展》2022年第3期174-179,共6页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究lncRNA SCAMP1靶向miR-197-3p对肝癌SNU-449细胞增殖和侵袭的影响。方法:qRT-PCR方法测定肝癌组织及细胞内SCAMP1表达变化。肝癌SNU-449细胞分成Control组、si-NC组(转染siRNA control)、si-SCAMP1组(转染SCAMP1 siRNA)组,CCK-8实验检测细胞增殖能力的变化,克隆形成实验测定各组细胞克隆能力的变化,Transwell小室检测各组细胞迁移和侵袭能力的变化,Western blot检测各组细胞中N-cadherin、E-cadherin蛋白表达水平的变化。在线靶基因软件预测SCAMP1的靶基因可能是miR-197-3p,经双荧光素酶报告系统鉴定两者之间的靶向关系。在肝癌SNU-449细胞中分别把SCAMP1 siRNA、miR-197-3p inhibitor共转染,利用上述方法检测细胞增殖、克隆、侵袭、迁移能力。结果:肝癌组织中SCAMP1表达水平高于癌旁组织,肝癌细胞内SCAMP1表达水平高于正常肝细胞,差异均有统计学意义(P<0.05)。与Control组、si-NC组比较,si-SCAMP1组肝癌SNU-449细胞增殖、克隆、侵袭、迁移能力均降低,细胞中N-cadherin蛋白表达水平降低,E-cadherin蛋白表达水平升高,差异均有统计学意义(P<0.05)。下调SCAMP1靶向促进miR-197-3p表达,差异有统计学意义(P<0.05)。miR-197-3p inhibitor能够逆转SCAMP1 siRNA对肝癌SNU-449细胞增殖、克隆、迁移和侵袭的抑制作用,差异均有统计学意义(P<0.05)。结论:下调SCAMP1靶向促进miR-197-3p降低肝癌SNU-449细胞增殖、克隆、迁移和侵袭能力。Objective:To study the effect of lncRNA SCAMP1 targeting miR-197-3p on the proliferation and invasion of hepatocellular carcinoma SNU-449 cells.Methods:The qRT-PCR method was used to determine the expression of SCAMP1 in hepatocellular carcinoma tissues and cells.Hepatocellular carcinoma SNU-449 cells were divided into Control group,si-NC group(transfection siRNA control),si-SCAMP1 group(transfection SCAMP1 siRNA)groups.The CCK-8 experiment detected the changes in cell proliferation,and the clone formation experiment determines the clonal ability of each group.Transwell chamber to detect the changes of cell migration and invasion ability of each group.Western blot to detect the changes of N-cadherin and E-cadherin protein expression levels in each group of cells.Online target gene prediction software predicted that the target gene of SCAMP1 may be miR-197-3p,and the target relationship between the two was identified by the luciferase reporter system.SCAMP1 siRNA and miR-197-3p inhibitor were co-transfected in hepatocellular carcinoma SNU-449 cells,respectively,and cell proliferation,cloning,invasion and migration were detected by the above method.Results:SCAMP1 expression level in hepatocellular carcinoma tissues was higher than that in adjacent tissues,and SCAMP1 expression level in hepatocellular carcinoma cells was higher than that in normal hepatocyte,with statistically significant differences(P<0.05).Compared with the control group and the si-NC group,the proliferation,cloning,invasion and migration of hepatocellular carcinoma SNU-449 cells in the si-SCAMP1 group were decreased,and the expression level of N-cadherin protein in the cells was decreased,while the expression level of E-cadherin protein was increased,with statistically significant differences(P<0.05).Down-regulation of SCAMP1 targeted to promote miR-197-3p expression,with statistically significant differences(P<0.05).miR-197-3p inhibitor could reverse the inhibitory effects of SCAMP1 siRNA on the proliferation,cloning,migration and invasion of he

关 键 词：SCAMP1 肝肿瘤 迁移 miR-197-3p 增殖 

分 类 号：R735.7[医药卫生—肿瘤]