胃癌中STIM1表达与其恶性程度及淋巴结转移的相关性研究  

Correlation between stromal interaction molecule 1 and tumor malignant degree or lymph node metastasis in patients with gastric cancer

作　　者：马波 姜淮芜 徐亮[1] 肖国 杨思雨 MA Bo;JIANG Huaiwu;XU Liang;XIAO Guo;YANG Siyu(Department of Gastrointestinal Surgery,The Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,P.R.China;Department of Gastrointestinal Surgery,The Sichuan Mianyang 404 Hospital,Mianyang,Sichuan 621000,P.R.China;Department of Pathology,The Sichuan Mianyang 404 Hospital,Mianyang,Sichuan 621000,P.R.China)

机构地区：[1]西南医科大学附属医院胃肠外科,四川泸州646000 [2]四川绵阳四〇四医院胃肠外科,四川绵阳621000 [3]四川绵阳四〇四医院病理科,四川绵阳621000

出　　处：《中国普外基础与临床杂志》2022年第4期465-469,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：2019年度绵阳市应用技术研究与开发项目(项目编号:2019YFZJ020)。

摘　　要：目的检测基质相互作用分子1(stromal interaction molecule 1,STIM1)在胃癌组织中的表达情况并分析其与胃癌恶性程度及淋巴结转移的相关性。方法收集2018年10月至2021年4月期间西南医科大学附属医院及四川绵阳四○四医院收治的83例胃癌患者。采用免疫组织化学染色方法检测胃癌组织及其相应的癌旁正常胃组织中STIM1蛋白表达情况,同时分析STIM1蛋白表达程度与胃癌患者临床病理学特征及术后淋巴结检出情况的关系。结果STIM1蛋白在胃癌组织中表达阳性率为95.2%(79/83),其中高表达组(STIM1评分5~12分)患者62例(74.7%)、低表达(STIM1评分2~4分)者21例(25.3%),而其在癌旁正常胃组织中蛋白表达阳性率为41.0%(34/83),STIM1蛋白表达阳性率在胃癌组织中高于其在癌旁正常胃组织(χ^(2)=58.078,P<0.001)。STIM1蛋白表达程度(高或低表达)与胃癌患者的性别、年龄及肿瘤大小均无关(P>0.05),而STIM1蛋白高表达者在肿瘤分化程度低/未分化、浸润深度T3+T4、TNM分期Ⅲ期及有淋巴结转移胃癌患者中占比高于肿瘤分化程度高/中分化(χ^(2)=11.052,P=0.001)、浸润深度T1+T2(χ^(2)=24.720,P<0.001)、TNM分期Ⅰ+Ⅱ期(χ^(2)=9.980,P=0.002)及无淋巴结转移(χ^(2)=6.097,P=0.014)者。STIM1蛋白表达强度与淋巴结转移数目(r=0.552,Z=–3.098,P=0.002)及淋巴结转移率(r=0.561,Z=–6.387,P<0.001)均呈正相关。结论STIM1蛋白在胃癌组织中的表达阳性率较高,胃癌组织中STIM1蛋白表达程度与肿瘤恶性程度及淋巴结转移密切相关,提示其可能在胃癌进展中发挥重要作用。Objective To investigate the correlation between expression of stromal interaction molecule 1(STIM1)and tumor malignant degree or lymph node metastasis in patients with gastric cancer.Methods A total of 83 patients with gastric cancer treated in the Affiliated Hospital of Southwest Medical University and Sichuan Mianyang 404 Hospital from October 2018 to April 2021 were collected.The expression of STIM1 protein in the gastric cancer tissues and the corresponding adjacent normal gastric tissues was detected by immunohistochemistry method.Meanwhile the correlation between the expression of STIM1 protein and clinicopathologic features or postoperative lymph node status of the patients with gastric cancer was analyzed.Results The positive rate of STIM1 protein expression in the gastric cancer tissues was 95.2%(79/83),including 62(74.7%)patients with high expression(STIM1 scoring 5–7)and 21(25.3%)patients with low expression(STIM1 scoring 2–4),which in the corresponding adjacent normal gastric tissues was 41.0%(34/83),the difference was statistically significant(χ^(2)=58.078,P<0.001).The expression of STIM1 protein was not related to gender,age,and tumor size of the patients with gastric cancer(P>0.05),while the proportions of the patients with high expression of STIM1 protein in the gastric cancer patients with low/undifferentiated tumor,T3+T4 of infiltration depth,TNM stageⅢ,and lymph node metastasis were higher than those with high/medium differentiation(χ^(2)=11.052,P=0.001),T1+T2 of infiltration depth(χ^(2)=24.720,P<0.001),TNM stageⅠ+Ⅱ(χ^(2)=9.980,P=0.002),and nonlymph node metastasis(χ^(2)=6.097,P=0.014).The expression intensity of STIM1 protein was positively correlated with the number of lymph node metastasis(r=0.552,Z=–3.098,P=0.002)and the rate of lymph node metastasis(r=0.561,Z=–6.387,P<0.001).Conclusions Positive rate of STIM1 protein expression in gastric cancer tissues is relatively high.STIM1 protein expression in gastric cancer tissue is closely related to tumor malignancy and lymph

关 键 词：胃癌 基质相互作用分子1 淋巴结转移 免疫组织化学法 

分 类 号：R735.2[医药卫生—肿瘤]