内皮细胞特异性分子1和血管内皮细胞生长因子在宫颈鳞癌中的表达及其与上皮-间质转化的相关性  

Expression of ESM-1 and VEGF in cervical squamous cell carcinoma and its correlation with EMT

作　　者：冯磊[1] 杨建敏[1] 杨然[1] 孙丽[1] 董莉丽 张清伟[2] FENG Lei;YANG Jianmin;YANG Ran;SUN Li;DONG Lili;ZHANG Qingwei(Department of Gynaecology,Nanyang Central Hospital,Nanyang,Henan 473000,China;Department of Gynaecology,the First Affiliated Hospital of Luohe Medical Vocational School,Luohe,Henan 462000,China)

机构地区：[1]南阳市中心医院妇科,河南南阳473000 [2]漯河医学高等专科学校第一附属医院妇科,河南漯河462000

出　　处：《安徽医药》2022年第5期969-973,共5页Anhui Medical and Pharmaceutical Journal

基　　金：河南省医学科技攻关计划项目(2018021026);河南省重点研发与推广专项(2020-LYZKYYB047)。

摘　　要：目的研究内皮细胞特异性分子1(ESM-1)和血管内皮细胞生长因子(VEGF)的表达在宫颈鳞癌中的关系及其与上皮-间质转化(EMT)的相关性。方法收集2018年12月至2019年12月在南阳市中心医院接受宫颈癌根治术的180宫颈鳞癌病例术后石蜡组织标本作为研究组,另外收集同期南阳市中心医院源于子宫肌瘤切除术的正常宫颈组织石蜡组织标本60例作为正常组,免疫组化检测石蜡组织标本中ESM-1、VEGF、E-钙黏蛋白(E-cadherin)和波形蛋白(Vimentin)的阳性表达率,比较正常宫颈和宫颈鳞癌组织中ESM-1、VEGF、E-Cadherin和Vimentin的阳性表达情况。比较其与宫颈鳞癌临床病理特征的关系,并分析ESM-1、VEGF与EMT相关蛋白E-Cadherin、Vimentin的相关性。结果免疫组化结果显示,ESM-1(81.7%比0.0%)、VEGF(66.7%比12.5%)、Vimentin(44.4%比0.0%)在宫颈鳞癌组织中的表达显著高于正常宫颈组织,E-Cadherin在宫颈鳞癌组织中的表达显著低于正常宫颈组织(45.6%比100.0%),差异有统计学意义(χ^(2)=40.00,P<0.05)。随着宫颈鳞癌FIGO分期越高,ESM-1和Vimentin阳性表达率越高,差异有统计学意义(χ^(2)=17.24,14.72,P<0.05),对VEGF和E-Cadherin阳性率的影响差异无统计学意义(χ^(2)=0.05,0.91,P>0.05);随着宫颈鳞癌分化程度越高,E-Cadherin和Vimentin阳性表达率越高,差异有统计学意义(χ^(2)=29.45,23.31,P<0.05),对ESM-1和VEGF阳性率的影响差异无统计学意义(χ^(2)=2.26,0.68,P>0.05);伴有淋巴结转移病人的ESM-1、VEGF及Vimentin阳性率高于不伴有淋巴结转移者,E-Cadherin阳性率低于不伴有淋巴结转移者,差异有统计学意义(χ^(2)=4.03,11.65,24.38,31.57,P<0.05);随着宫颈鳞癌宫颈浸润深度的增加,ESM-1和VEGF阳性表达率升高,差异有统计学意义(χ^(2)=6.87,12.92,P<0.05),对E-Cadherin和Vimentin阳性率的影响差异无统计学意义(χ^(2)=1.58,1.53,P>0.05)。宫颈组织中,ESM-1和VEGF的表达与E-Cadherin呈负相关(r=−0.46,−0.31,P<0Objective To study the relationship between the expression of membrane cell specific molecule-1(ESM-1)and vascular cell growth factor(VEGF)in cervical squamous cell carcinoma and its correlation with epithelial-mesenchymal transition(EMT).Methods The postoperative paraffin tissue specimens of 180 cases of cervical squamous cell carcinoma who underwent radical resection of cervical cancer in the Nanyang Central Hospital from December 2018 to December 2019 was collected as the research group,60 cases of normal cervical tissue paraffin tissue specimens in the Nanyang Central Hospital were regarded as the normal group at the same period.The immunohistochemical was used to detect the positive expression rate of ESM-1,VEGF,E cadherin and Vimentin in paraffin tissue samples.The positive expression of ESM-1,VEGF,E cadherin and Vimentin in normal cervix and cervical squamous carcinoma tissues was analyzed.The relationship between ESM-1,VEGF,E-Cadherin and Vimentin and clinicopathological features of cervical squamous cell carcinoma was compared,and the correlation between ESM-1,VEGF and EMT-related proteins E-Cadherin and Vimentin was analyzed.Results Immunohistochemical results showed that the expression of ESM-1(81.7%vs.0.0%),VEGF(66.7%vs.12.5%)and Vimentin(44.4%vs.0.0%)in cervical squamous cell carcinoma tissues was significantly higher than that in normal cervical squamous cell carcinoma tissues,and the expression of E-Cadherin in cervical squamous cell carcinoma tissues was significantly lower than that in normal cervical squamous cell carcinoma tissues,with statistically significant differences(χ^(2)=40.00,P<0.05).The higher FIGO stage of cervical squamous cell carcinoma,the higher positive expression rates of ESM-1 and Vimentin,the difference was statistically significant(χ^(2)=17.24,14.72,P<0.05),and there was no statistically significant difference in the positive rates of VEGF and E-Cadherin(χ^(2)=0.05,0.91,P>0.05).The higher the differentiation degree of cervical squamous cell carcinoma,the higher the positi

关 键 词：宫颈肿瘤 癌 鳞状细胞 上皮-间质转化 内皮细胞特异性分子1 血管内皮细胞生长因子 

分 类 号：R737.33[医药卫生—肿瘤]