二氢杨梅素下调大鼠缺血性脑卒中脑组织炎症小体Nod样受体蛋白3的表达  

Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats

作　　者：丁慧如 邓心未 刘怀存 程全成 方璇 张国梁[2] 陈春花 张卫光[1] DING Hui-ru;DENG Xin-wei;LIU Huai-cun;CHENG Quan-cheng;FANG Xuan;ZHANG Guo-liang;CHEN Chun-hua;ZHANG Wei-guang(Department of Human Anatomy,Histology and Embryology,School of Basic Medical Sciences,Peking University,Beijing 100083,China;Department of Neurobiology,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]北京大学基础医学院人体解剖学与组织学胚胎学系,北京100083 [2]河北医科大学神经生物学研究室,石家庄050017

出　　处：《解剖学报》2022年第2期137-143,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(81873769)。

摘　　要：目的 研究二氢杨梅素(DHM)在大鼠缺血性脑卒中治疗方面的作用,并探讨其对炎症小体Nod样受体蛋白3(NLRP3)表达的影响。方法 取70只SD大鼠,利用线栓法构建大鼠大脑中动脉栓塞(MCAO)模型,采用Longa评分、TTC染色、Nissl染色、免疫组织化学染色及Western blotting等方法,探讨DHM对MCAO大鼠缺血/再灌注损伤的治疗效果及机制。结果 DHM治疗后MCAO大鼠自主运动能力明显改善,脑组织梗死体积明显减小,脑组织结构和神经元形态较为改善,且DHM治疗后的NLRP3和炎症因子白细胞介素1β(IL-1β)蛋白表达显著降低。结论 DHM能下调炎症小体的表达,进而降低MCAO脑卒中大鼠的脑梗死体积并改善神经行为学表现。Objective To investigate the role of dihydromyricetin(DHM) in the treatment of ischemic stroke in rats, and to explore the effect of DHM on the expression of inflammasome. Methods The middle cerebral artery occlusion(MCAO) model was induced by endovascular suture method. The therapeutic effect and mechanism of DHM were investigated by Longa score, TTC staining, Nissl staining, immunohistochemical staining and Western bloting. Results After DHM treatment, the motor capacity of MCAO rats was significantly improved, the infarct volume was significantly reduced, the brain structure and neuron morphology were improved, and the expressions of nod-like receptor protein-3(NLRP3) and interleukin-1β(IL-1β) decreased significantly. Conclusion DHM can down-regulate the expression of NLRP3 and thus reduces the cerebral infarction volume and improves neurobehavioral performance in MCAO rats.

关 键 词：缺血性脑卒中 大脑中动脉栓塞 二氢杨梅素 炎症小体 Nod样受体蛋白3 TTC染色 Longa评分 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]