4种多环芳烃对仿刺参耗氧率和排氨率的影响研究  

Effect of four distinct PAHs on the oxygen consumption and the ammonia exhalation rate of Apostichopus japonicus

作　　者：刘帅 詹泽荣 车鉴 颉海波 魏海峰 李正炎[1,2] 刘长发 LIU Shuai;ZHAN Ze-rong;CHE Jian;JIE Hai-bo;WEI Hai-feng;LI Zheng-yan;LIU Chang-fa(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Key Laboratory of Marine Environment and Ecology of Ministry of Education,Ocean University of China,Qingdao 266100,China;Key Laboratory of Coastal Marine Environmental Science and Technology of Liaoning Province,Dalian 116023,China;Dalian Ocean University,School of Marine Science and Environment Engineering,Dalian 116023,China;Technology Innovation Center for Coastal Ecological Environment and Disaster Protection,Dalian 116023,China;Dalian Xinyulong Marine Biological Seed Technology Co.,Dalian 116222,China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [3]辽宁省近岸海洋环境科学与技术重点实验室,辽宁大连116023 [4]大连海洋大学海洋科技与环境学院,辽宁大连116023 [5]辽宁省近海生态环境与灾害防护工程技术创新中心,辽宁大连116023 [6]大连鑫玉龙海洋生物种业科技股份有限公司,辽宁大连116222

出　　处：《海洋科学》2022年第4期106-113,共8页Marine Sciences

基　　金：国家重点研发项目(2018YFC1407600);辽宁省教育厅服务地方项目(DL201804);国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金项目(201309,201809);国家海洋局近岸海域生态环境重点实验室基金项目(201013);大连海洋大学农业农村部北方海水增养殖重点实验室基金项目(2018-KF-21)。

摘　　要：为探究多环芳烃(PAHs)对海洋生物的生态毒理效应,作者将仿刺参(Apostichopus japonicus)分别暴露于质量浓度为10、20、50、100、150μg/L的菲、三甲基菲、蒽和二甲基蒽中,检测4种代表性多环芳烃胁迫下仿刺参的耗氧率和排氨率。结果显示:在不同质量浓度多环芳烃的胁迫下,仿刺参的耗氧率均显著高于对照组,且单位体质量仿刺参耗氧率受三甲基菲浓度影响最大,其相关方程为y=4.8971x+1.2267,R^(2)=0.8945;单位体质量仿刺参排氨率随4种多环芳烃浓度的增加出现上下波动的现象,且在菲胁迫下,其排氨率波动幅度最大,在菲质量浓度为20μg/L时达到最大值22.87μg/(g·h);O︰N比在4种不同浓度多环芳烃的作用下均显著高于对照组并整体随暴露浓度呈上升趋势,且受二甲基蒽浓度影响最大,其相关方程为y=0.3471x+0.3967,R^(2)=0.8435。上述实验结果为多环芳烃类污染物对仿刺参的毒性作用机制探究提供了科学依据。To investigate the toxic effects of PAHs,which is mainly composed of phenanthrene,3-methylphenan-threne,anthracene,and 2-methylanthracene on the marine organisms,the sea cucumber Apostichopus japonicus was introduced to PAHs at varied concentrations of 10,20,50,100 and 150μg/L,respectively.The oxygen con-sumption rate and the ammonia excretion rate of A.japonicus under the stress of four distinct PAHs were meas-ured accordingly.The results demonstrated that under the stress of four respective concentrations of PAHs,the oxygen consumption rate of A.japonicus was significantly higher than that of the control group,and the concen-tration of 3-methylphenanthrene had the greatest influence on the oxygen consumption rate per unit body weight,with the correlation equation of y=4.8971x+1.2267,R^(2)=0.8945.Similarly,the ammonia emission per unit body weight also fluctuated with the increase of PAH concentration.Under the phenanthrene stress,with the phenanthrene concentration of 20μg/L,the ammonia exhalation rate fluctuated the most and reached the maxi-mum of 22.87μg/(g·h).The O:N ratio was substantially higher than those of the control group under the four distinct concentrations of PAHs,and proliferated with the overall exposure concentration,and was most affected by the concentration of 2-methylanthracene with the correlation equation of y=0.3471x+0.3967,R^(2)=0.8435.The experimental results established a scientific rationale for exploring the toxic mechanism of PAHs on A.ja-ponicus.

关 键 词：多环芳烃 仿刺参(Apostichopus japonicus) 耗氧率 排氨率 O︰N比 

分 类 号：X171.5[环境科学与工程—环境科学]