Engineering nano-structures with controllable dimensional features on micro-topographical titanium surfaces to modulate the activation degree of M1 macrophages and their osteogenic potential  

作　　者：Luxin Liang Qianli Huang Hong Wu Hao He Guanghua Lei Dapeng Zhao Kun Zhou 

机构地区：[1]State Key Laboratory of Powder Metallurgy,Central South University,Changsha 410083,China [2]Foshan(Southern China)Institute for New Materials,Foshan 528200,China [3]Department of Vascular Surgery,The Second Xiangya Hospital,Central South University,Changsha 410011,China [4]Department of Orthopedics,Xiangya Hospital,Central South University,Changsha 410008,China [5]College of Biology,Hunan University,Changsha 410082,China [6]School of Mechanical and Aerospace Engineering,Nanyang Technological University,Singapore 639798,Singapore

出　　处：《Journal of Materials Science & Technology》2022年第1期167-178,共12页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.51771233,52071346 and 51604104);China Postdoctoral Science Foundation(Grant No.2018M633164);Innovation-oriented Advanced Technology and Industrial Technology Program Project of Hunan Province(Grant No.2020SK2017);Guangdong Basic and Applied Basic Research Foundation(Grant No.2019A1515110736)。

摘　　要：Modulating the activation state and degree of macrophages still remains as a challenge for the topographical design of Ti-based implants.In this work,micro/nano-structured coatings were prepared on Ti substrates by micro-arc oxidation(MAO)and subsequent hydrothermal(HT)treatment.By varying the HT conditions,plate-like nano-structures with an average length of 80,440 or 780 nm were obtained on MAO-prepared micro-topographical surfaces.Depending on the dimensional features of nano-plates,the specimens were noted as Micro,Micro/Nano-180,Micro/Nano-440 and Micro/Nano-780,respectively.The in vitro results showed that the activation state and degree of macrophages could be effectively modulated by the micro/nano-structured surfaces with various dimensional features.Compared to the Micro surface,the Micro/Nano-180 surface activated both M1 and M2 phenotype in macrophages,while the Micro/Nano-440 and Micro/Nano-780 surfaces polarized macrophages to their M1 phenotype.The activation degree of M1 macrophages followed the trend:Micro<Micro/Nano-180<Micro/Nano-440<Micro/Nano-780.However,the osteogenic potential of the activated macrophages in response to various surfaces were in the order:Micro≈Micro/Nano-780<Micro/Nano-180<Micro/Nano-440.Together,the findings presented in this work indicate that engineering nano-structures with controllable dimensional features is a promising strategy to modulate macrophage activation state and degree.In addition,it is essential to determine the appropriate activation degree of M1 macrophages for enhanced osteogenesis.

关 键 词：Titanium Micro/nano-structures Macrophages activation Inflammatory response OSTEOGENESIS 

分 类 号：R318.08[医药卫生—生物医学工程] TG174.4[医药卫生—基础医学]