circ_0001588靶向miR-1286调控人胃癌AGS细胞糖酵解、增殖、迁移及侵袭  

circ_001588 Regulates Glycolysis,Proliferation,Migration and Invasion of Gastric Cancer AGS Cells by Targeting miR-1286

作　　者：吴萌 张洁[3] 周思敏 王思普 李莹[3] 周璐[3] 张庆瑜[3] 王邦茂[3] 姜葵[3] Wu Meng;Zhang Jie;Zhou Simin(Graduate School of Tianjin Medical University,Tianjin 300070,China;Department of Gastroenterology,Beidaihe Hospital,Qinhuangdao 066100,China;Department of Gastroenterology,General Hospital of Tianjin Medical University,Tianjin300070,China)

机构地区：[1]天津医科大学研究生院,天津300070 [2]河北省秦皇岛市北戴河医院消化科,秦皇岛066100 [3]天津医科大学总医院消化内科,天津300070

出　　处：《华中科技大学学报（医学版）》2022年第2期180-186,共7页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：2019年天津市科技局重大疾病防治科技重大专项(No.19ZXDBSY00020)。

摘　　要：目的探讨circ_0001588对人胃癌AGS细胞糖酵解、增殖、迁移及侵袭的影响及可能机制。方法采用实时荧光定量PCR技术(qRT-PCR)检测胃癌组织、癌旁组织、人胃癌细胞株中circ_0001588、miR-1286表达;将人胃癌细胞分为si-NC组、si-circ_0001588组、miR-NC组、miR-1286 mimics组、si-circ_0001588+anti-miR-NC组、si-circ_0001588+anti-miR-1286组;利用试剂盒检测细胞葡萄糖消耗、乳酸生成;采用CCK-8法、平板克隆形成实验检测细胞增殖能力;划痕实验与Transwell实验检测细胞迁移及侵袭;双荧光素酶报告基因实验检测circ_0001588与miR-1286的靶向关系;采用Western blot检测E-cadherin、N-cadherin蛋白表达。结果与癌旁组织比较,胃癌组织中circ_0001588表达升高(P<0.05),miR-1286表达降低(P<0.05);不同分型、不同浸润深度、是否远处转移和不同临床分期患者胃癌组织circ_0001588、miR-1286表达存在差异(均P<0.05);人胃癌细胞AGS、SGC-7901和BGC823较正常胃黏膜细胞的circ_0001588表达升高,miR-1286表达降低(均P<0.05),以AGS细胞最为显著;与si-NC组比较,si-circ_0001588组葡萄糖消耗、乳酸生成、细胞活力、细胞克隆形成数、划痕愈合率、侵袭细胞数和N-cadherin蛋白表达均降低(均P<0.05),E-cadherin蛋白表达增高(P<0.05);与miR-NC组比较,miR-1286 mimics组葡萄糖消耗、乳酸生成、细胞活力、细胞克隆形成数、划痕愈合率、侵袭细胞数和N-cadherin蛋白表达均降低(均P<0.05),E-cadherin蛋白表达增高(P<0.05);与si-circ_0001588+anti-miR-NC组比较,si-circ_0001588+anti-miR-1286组葡萄糖消耗、乳酸生成、细胞活力、细胞克隆形成数、划痕愈合率、侵袭细胞数和N-cadherin蛋白表达均增高(均P<0.05),E-cadherin蛋白表达降低(P<0.05)。结论干扰circ_0001588表达可通过靶向调控miR-1286而抑制胃癌细胞糖酵解、增殖、克隆形成、迁移及侵袭。Objective To explore the effect of circ_0001588 on glycolysis,proliferation,migration and invasion of gastric cancer AGS cells and its possible mechanism.Methods The real time quantitative PCR(qRT-PCR)was used to detect the expression of circ_0001588 and miR-1286 in gastric cancer tissues,adjacent tissues,human gastric cancer cells AGS,SGC-7901 and BGC823.Human gastric cancer cells were divided into si-NC group,si-circ_0001588 group,miR-NC group,miR-1286 mimics group,si-circ_0001588+anti-miR-NC group,and si-circ0001588+anti-miR-1286 group.Glucose consumption and lactic acid production levels were detected by specific kits.CCK-8 method,plate clone formation test,scratch test and Transwell test were used to detect cell proliferation,clone formation,migration and invasion,respectively.The dual-luciferase reporter experiment was used to detect the targeting relationship between circ_0001588 and miR-1286.Western blotting was used to detect the expression of E-cadherin and N-cadherin protein.Results Compared with adjacent tissues,the expression of circ_0001588 in gastric cancer tissues was significantly increased(P<0.05),while the expression of miR-1286 was decreased(P<0.05).There were differences in the expressions of circ_0001588 and miR-1286 in gastric cancer tissues of patients with different cancer types,different depths of invasion,distant metastasis and different clinical stages(all P<0.05).In human gastric cancer cells AGS,SGC-7901 and BGC823,the expression of circ_0001588 was increased and miR-1286 was decreased compared with normal cells,among which AGS had the highest expression of circ_0001588 and the lowest expression of miR-1286(all P<0.05).Human gastric cancer cells AGS were selected for further research.Compared with si-NC group,glucose consumption,lactate,cell viability,number of cell clones,scratch healing rate,number of invasive cells and N-cadherin protein level were all decreased in si-circ_0001588 group(all P<0.05),while E-cadherin were increased in si-circ_0001588 group(P<0.05).Compared with miR-

关 键 词：胃癌 circ_0001588 miR-1286 糖酵解 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]