Circ_0000502靶向调节miR-1231促进口腔鳞状细胞癌HSC-3细胞增殖和抑制细胞凋亡的体外研究  

Circ_0000502 promotes the proliferation and inhibites apoptosis of oral squamous cell carcinoma HSC-3 cells by targeting miR-1231 in vitro

作　　者：马素伟 张娟 杨晋[1] 靳昊[1] 王焱[1] 高杰[1] MA Suwei;ZHANG Juan;YANG Jin;JIN Hao;WANG Yan;GAO Jie(Baoding Municipal 2nd Central Hospital,Baoding 072750,Hebei Province,China)

机构地区：[1]保定市第二中心医院,河北保定072750

出　　处：《口腔颌面外科杂志》2022年第2期93-99,共7页Journal of Oral and Maxillofacial Surgery

摘　　要：目的:探讨环状RNA 0000502(circ_0000502)是否靶向微小RNA-1231(microRNA-1231,miR-1231)调控口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)细胞HSC-3的增殖和凋亡。方法:实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)分析OSCC组织、癌旁组织中circ_0000502和miR-1231的表达水平。四甲基偶氮唑盐微量酶反应比色法(MTT法)、流式细胞术分析circ_0000502和miR-1231表达对HSC-3细胞增殖和凋亡的影响。双荧光素酶报告实验和RT-qPCR用于确定circ_0000502和miR-1231调控关系。结果:OSCC组织中,circ_0000502呈高表达,miR-1231呈低表达(P<0.05)。抑制circ_0000502或过表达miR-1231能显著降低HSC-3的细胞活力,提高细胞凋亡率(P<0.05)。与单独抑制circ_0000502比较,同时抑制circ_0000502和miR-1231后,HSC-3细胞活力显著升高,凋亡率显著降低(P<0.05)。结论:circ_0000502靶向负调控miR-1231能促进OSCC细胞HSC-3增殖,抑制细胞凋亡。Circ_0000502/miR-1231分子轴是OSCC的潜在治疗靶点。Objective:In this article,we explore whether circular RNA 0000502(circ_0000502)targets miR-1231 and regulates the proliferation and apoptosis of oral squamous cell carcinoma(OSCC)cell line HSC-3.Methods:The expression levels of circ_0000502 and miR-1231 in OSCC tissues and adjacent tissues were analyzed by real-time quantitative polymerase chain reaction(RT-qPCR).MTT method and flow cytometry were used to analyze the effects of circ_0000502 and miR-1231 expression on the proliferation and apoptosis of HSC-3.The dual luciferase reporter assay and RT-qPCR were performed to determine the regulatory relationship between circ_0000502 and miR-1231.Results:Circ_0000502 was intensely expressed and miR-1231 was weakly expressed in OSCC tissues(P<0.05).Inhibition of circ_0000502 or overexpression of miR-1231 significantly reduced HSC-3 cell viability and increased cell apoptosis rate(P<0.05).Compared with the inhibition of circ_0000502,the cell viability of HSC-3 cells was significantly increased,and the apoptosis rate was significantly reduced after both circ_0000502 and miR-1231 were inhibited(P<0.05).Conclusion:The above findings suggest that circ_0000502 promotes the proliferation and inhibits cell apoptosis of OSCC HSC-3 cells by negatively regulating miR-1231.The circ_0000502/miR-1231 molecular axis is a potential therapeutic target for OSCC.

关 键 词：环状RNA 0000502 微小RNA-1231 口腔鳞状细胞癌 细胞增殖 凋亡 

分 类 号：R782[医药卫生—口腔医学]