植物乳杆菌P9对头孢曲松钠作用小鼠肠道菌群的影响  

Effect of Lactobacilus plantarum P9 on the Intestinal Flora of Mice Treated with Ceftriaxone Sodium

作　　者：陈苗苗 李可文 熊雪梅 吴梦琦 刘小溪 郑秋生[1] CHEN Miao-miao;LI Ke-wen;XIONG Xue-mei;WU Meng-qi;LIU Xiao-xi;ZHENG Qiu-sheng(College of Pharmacy,Shihezi University,Shihezi 832000,Xinjiang,China;College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;College of Biochemical Engineering,Beijing Union University,Beijing 100023,China)

机构地区：[1]石河子大学药学院,新疆石河子832000 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030 [3]北京联合大学生物化学工程学院,北京100023

出　　处：《食品研究与开发》2022年第8期16-27,共12页Food Research and Development

基　　金：国家自然科学基金(31870338);山东省优势学科团队支持计划(2016052410)。

摘　　要：为了探究植物乳杆菌P9对抗生素致肠道菌群失调小鼠肠道菌群的影响,建立小鼠肠道菌群失调模型,再灌胃不同剂量植物乳杆菌P9菌粉,酶联免疫吸附法测定血清中炎症细胞因子水平,可见分光光度法测定肝脏和小肠组织中抗氧化指标,菌群计数和16S rDNA高通量测序探究小鼠肠道菌群的变化。结果显示,植物乳杆菌P9能够降低小鼠因抗生素造成的炎症反应,提高脏器氧化应激水平。菌群计数结果显示植物乳杆菌P9能够增加抗生素导致的双歧杆菌、乳酸杆菌的减少。16S r DNA测序结果显示,抗生素使小鼠肠道对抗生素耐药细菌丰度增加,而植物乳杆菌P9则降低耐药细菌丰度,植物乳杆菌P9具有调节小鼠肠道菌群的作用。The purpose of the experiment was to explore the effect of Lactobacilus plantarum P9 on the intestinal flora of mice treated with ceftriaxone sodium. The experiment modeled the intestinal flora imbalance in mice after antibiotic treatment and then administered different doses of Lactobacillus plantarum P9 powder. The levels of inflammatory cytokines in the serum were analyzed using enzyme-linked immunosorbent assays. The antioxidant index in the liver and small intestine were identified using visible spectrophotometry. Bacterial counting and 16S rDNA high-throughput sequencing investigated any changes in the intestinal flora of the mice. The results revealed Lactobacillus plantarum P9 can reduce the inflammatory responses to antibiotics and suppress the oxidative stress in organs. Flora counts revealed Lactobacillus plantarum P9 could increase the number of Bifidobacteria and Lactobacillus,limiting the decrease caused by antibiotic use. The 16S rDNA sequencing showed an increase in the abundance of antibiotic-resistant bacteria in the antibiotic treated mice intestines,adding Lactobacillus plantarum P9 reduced the prevalence of drug-resistant bacteria. In summary,Lactobacillus plantarum P9 effectively regulates mice intestinal flora.

关 键 词：植物乳杆菌P9 头孢曲松钠 肠道菌群 抗生素 16S rDNA测序 

分 类 号：TS201.3[轻工技术与工程—食品科学]