丁香不同部位挥发油的GC-MS成分分析和抗氧化活性比较  

GC-MS Analysis and Comparison of Antioxidant Activities of Volatile Oils from Different Parts of Eugenia caryophyllata Thunb.

作　　者：左遨勋 刘积光 高玉梅 刘平怀[1] ZUO Ao-xun;LIU Ji-guang;GAO Yu-mei;LIU Ping-huai(Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]海南大学,海南海口570228

出　　处：《食品研究与开发》2022年第8期146-151,共6页Food Research and Development

基　　金：国家科技支撑计划项目(2011BAD14B01);国家科技型中小企业技术创新基金项目(13C26244604892);海南省产学研一体化项目(CXY20150034);海南省中药现代化科技专项(ZY201327);两种药食同源南药资源的综合利用价值研究(ZDYF2020169)。

摘　　要：通过水蒸气蒸馏法提取丁香不同部位(花蕾、果实、叶、枝)的挥发油,并采用气相色谱-质谱法鉴定其化学成分组成,并研究其抗氧化活性。结果表明,丁香酚、乙酰丁香酚和β-石竹烯等是丁香4个部位的主要成分;丁香花蕾、果实、叶、枝部位挥发油对DPPH·的IC;值分别为(37.35±0.82)、(98.18±0.50)、(21.37±0.32)、(28.14±0.46)μg/mL;对ABTS;·的IC;值为(139.48±0.76)、(244.99±1.21)、(117.32±0.63)、(141.61±0.84)μg/m L;还原力和铁离子还原/抗氧化能力(ferric ion reducing antioxidant power,FRAP)测定结果:丁香叶最大,花蕾与枝数值近似,丁香果实最小。The volatile oils of Eugenia caryophyllata Thunb. were extracted from buds,fruits,leaves,and branches by steam distillation. Their chemical compositions were identified by gas chromatography-mass spectrometry(GC-MS),and antioxidant activities were investigated. The results showed that eugenol,acetyleugenol,and β-caryophyllene served as the main components of the four parts of E. caryophyllata. The IC;values of volatile oils from buds,fruits,leaves,and branches on DPPH·were(37.35±0.82),(98.18±0.50),(21.37±0.32)μg/mL,and(28.14±0.46)μg/mL,respectively,while the IC;values on ABTS;·were(139.48±0.76),(244.99±1.21),(117.32±0.63)μg/mL,and(141.61±0.84)μg/mL,respectively. Additionally,as revealed by reducing power and ferric ion reducing antioxidant power(FRAP)assays,the value in the leaves was the highest,followed by those in the buds and branches,and that in the fruits was the lowest.

关 键 词：丁香 挥发油 化学成分 丁香酚 抗氧化活性 

分 类 号：O657.63[理学—分析化学] TQ654.2[理学—化学]