新型植物提取物添加剂对大口黑鲈生长、抗氧化及糖代谢影响的研究  

Effects of Plant Extracts Feed Additives on Growth,Antioxidant and Glucose Metabolism of Largemouth Bass

作　　者：张小雨 高彬 徐田田 韩敏祯 李松林 吕利群[1,2,3,4] 许丹[1,2,3,4] 刘兴旺 ZHANG Xiaoyu;GAO Bin;XU Tiantian;HAN Minzhen;LI Songlin;LYU Liqun;XU Dan;LIU Xingwang(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Aquaculture Ministry for Freshwater Aquatic Genetic Resources,Shanghai Ocean University,Shanghai 201306,China;National Experimental Teaching Demonstration Center for Fishery Sciences,Shanghai Ocean University,Shanghai 201306,China;Shanghai Moryo Technology Co.,Ltd.,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海海洋大学国家水生动物病原体采集中心,上海201306 [3]上海海洋大学水产养殖部淡水水生遗传资源重点实验室,上海201306 [4]上海海洋大学水产科学实验教学示范中心,上海201306 [5]上海牧渔科技有限公司,上海201306

出　　处：《饲料工业》2022年第8期29-34,共6页Feed Industry

基　　金：上海海洋大学协同创新项目[A1-3316-20-0000];上海市人才发展资金资助计划[2019097]。

摘　　要：为评估新型植物提取物添加剂对大口黑鲈(Micropterus salmoides)生长、抗氧化及糖代谢的影响,配制基础饲料并添加0、1500 mg/kg和3000 mg/kg的新型植物提取物添加剂以生产3种试验饲料[S1(对照组)、S2组和S3组],分别投喂3组(共225尾)初始重量为(7.50±0.23)g的大口黑鲈8周。结果显示:饲料中添加新型植物提取物添加剂显著提高了大口黑鲈增重率(WGR)和存活率,并降低了饲料系数(FCR)(P<0.05)。血浆总蛋白(TP)、总胆固醇(TC)、三酰甘油(TG)含量随新型植物提取物添加剂添加而升高,S3组显著高于对照组和S2组(P<0.05),低密度脂蛋白(LDL)和白蛋白(ALB)S3组显著高于对照组(P<0.05)。肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性随新型植物提取物添加剂添加量升高显著下降(P<0.05)。肝脏炎症因子白细胞介素10(IL-10)和1β(IL-1β)在S2组表达量显著上调(P<0.05),肿瘤坏死因子α(TNF-α)表达量在S3组显著下调(P<0.05)。肝脏过氧化物酶体增殖物激活受体α(PPARα)、脂肪酸合酶(FAS)表达量在S2组显著上调(P<0.05)。肝脏葡萄糖激酶(GK)、己糖激酶(HK)和6磷酸葡萄糖脱氢酶(G6PDH)表达量在S2组显著上调(P<0.05)。丙酮酸激酶(PK)、葡萄糖-6-磷酸脱氢酶(G6Pase)和磷酸烯醇式丙酮酸激酶(PEPCK)的表达量则随新型植物提取物添加剂添加而显著升高(P<0.05)。综上所述,新型植物提取物添加剂能够显著提高大口黑鲈的存活率和生长性能,通过影响糖酵解途径的GK、HK、PK、增强G6Pase的表达降低大口黑鲈糖原积累,从而减轻大口黑鲈的肝糖应激。To assess the effects of plant extracts feed additives on the growth,antioxidant and glucose metabolism of largemouth bass,three experimental diets were formulated with 0,1 500 mg/kg and 3 000 mg/kg of plant extracts feed additives supplementation to feed three groups(S1,S2 and S3 group) of largemouth bass (225 fishes intotal) with an initial weight of(7.50±0.23) g for eight weeks respectively.The resultsshowed that the addition of plant extracts feed ad-ditives to the diet significantly increased theweight gain rate(WGR),and survival rate of large-mouth bass,and reduced the bait coefficient(FCR)(P<0.05).Plasma total protein(TP),total cholesterol(TC) and triglycerides(TG) were increased with the addition of plant extracts feed additives,and those in S3 group were significantly higher than those in the control and S2 groups(P<0.05),low den-sity lipoprotein(LDL) and albumin(ALB) in S3 group were significantly higher than those in controlgroup(P<0.05).Hepatic superoxide dismutase(SOD) and catalase(CAT) activities decreased significantly(P<0.05) with the increase of plant extracts feed additives addition.Hepatic inflammatory factors interleu-kin IL-10 and 1β(IL-1β) expression were significantly upregulated in the S2 group(P<0.05),and tumournecrosis factor alpha(TNF-α) expression was significantly downregulated in the S3 group(P<0.05).Liverperoxisome proliferator-activated receptor alpha(PPARα) and fatty acid synthase(FAS) expression weresignificantly upregulated in S2 group(P<0.05).Hepatic glucokinase(GK),hexokinase(HK) and glucose 6 phosphate dehydrogenase(G6 PDH) expression were significantly upregulated in S2 group(P<0.05).Theexpression of pyruvate kinase(PK),glucose-6-phosphate dehydrogenase(G6 Pase) and phosphoenolpyru-vate kinase(PEPCK),on the other hand,were significantly increased with the addition of plant extractsfeed additives(P<0.05).In summary,plant extracts feed additives significantly improved the survival andgrowth performance of Californian bass and reduced liver sugar stress in largemouth bass

关 键 词：大口黑鲈 生长 抗氧化能力 肝糖应激 糖代谢 

分 类 号：S816.7[农业科学—饲料科学]