过表达BTAF2对肝癌Bel-7402细胞侵袭和迁移的影响  

Effect of over-expression of BATF2 on invasion and migration of hepatoma Bel-7402 cells

作　　者：张畅 周浮杨 郑宇航 魏超越 王璎珞 孙艳[2] Zhang Chang;Zhou Fuyang;Zheng Yuhang;Wei Chaoyue;Wang Yingluo;Sun Yan(School of basic medical science,Qiqihar Medical University,Qiqihar,Heilongjiang,161000,China;school of medical technology,Qiqihar Medical University,Qiqihar,Heilongjiang,161000,China)

机构地区：[1]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161000 [2]齐齐哈尔医学院医学技术学院,黑龙江齐齐哈尔161000

出　　处：《齐齐哈尔医学院学报》2022年第4期301-303,共3页Journal of Qiqihar Medical University

基　　金：黑龙江省大学生创新创业训练计划项目(201911230002)。

摘　　要：目的 探讨碱性亮氨酸拉链转录因子2(BATF2)过表达对肝癌Bel-7402细胞侵袭迁移的影响。方法 利用Lipofeetamine 3000 Reagent脂质体转染法进行肝癌Bel-7402细胞转染,以转染pcDNA3.1空载体的Bel-7402细胞和转染pcDNA3.1-BATF2的Bel-7402细胞为研究对象,利用Western blot法检测转染后Bel-7402细胞的BATF2蛋白表达水平,Transwell小室检测转染后Bel-7402细胞侵袭能力,细胞划痕实验检测转染后Bel-7402细胞迁移能力。结果 相较于pcDNA3.1组,pcDNA3.1-BATF2组蛋白表达量显著增加,差异具有显著性(P<0.05);pcDNA3.1组侵袭穿膜的细胞数为(108.5±27.7)个,而pcDNA3.1-BATF2组侵袭穿膜的细胞术为(45.6±17.9)个,pcDNA3.1-BATF2组细胞穿膜数量明显减少(P<0.05);pcDNA3.1组迁移距离为(90.15±27.58)μm,而pcDNA3.1-BATF2组迁移距离为(51.46±16.17)μm, pcDNA3.1-BATF2组细胞迁移距离明显缩短(P<0.05)。结论 BATF2过表达能够抑制肝癌细胞Bel-7402侵袭和迁移能力。Objective To investigate the effects of over-expression of basic leucine zipper transcription factor 2(BATF2) on the invasion and migration of hepatoma Bel-7402 cells.Methods The Lipofeetamine 3000 Reagent liposome transfection method was used to transfect liver cancer Bel-7402 cells. Bel-7402 cells transfected with pcDNA3.1 empty carrier and Bel-7402 cells transfected with pcDNA3.1-BATF2 were taken as research objects. The expression level of BATF2 protein in transfected Bel-7402 cells was detected by Western blot, the invasive ability of transfected Bel-7402 cells was detected by Transwell chamber, and the migration ability of transfected Bel-7402 cells was detected by cell scratch test.Results Compared with the pcDNA3.1 group, the histone expression of pcDNA3.1-BATF2 was significantly increased and the difference was statistically significant(P<0.05). The number of cells penetrating the membrane in the pcDNA3.1 group was(108.5±27.7), while that in the pcDNA3.1-BATF2 group was(45.6±17.9). The number of cells penetrating the membrane in the pcDNA3.1-BATF2 group was significantly decreased(P<0.05). The migration distance in the pcDNA3.1 group was(90.15±27.58) μm, while that in the pcDNA3.1-BATF2 group was(51.46±16.17)μm.The cell migration distance in the pcDNA3.1-BATF2 group was significantly shortened(P<0.05).Conclusions Over-expression of BATF2 could inhibit the invasion and migration of hepatoma cell Bel-7402.

关 键 词：BATF2 肝癌 侵袭 迁移 

分 类 号：R735.7[医药卫生—肿瘤]