复合人工湿地系统对低污染水总氮的净化效果及其微生物群落结构特征  

PURIFICATION EFFECT OF TOTAL NITROGEN IN LOW-POLLUTED WATER BY A COMPOSITE CONSTRUCTED WETLAND SYSTEM AND ITS MICROBIAL COMMUNITY STRUCTURE

作　　者：余俊霞 陈双荣 刘凌言 李莹美 陆轶峰[2] 马兰 YU Junxia;CHEN Shuangrong;LIU Lingyan;LI Yingmei;LU Yifeng;MA Lan(Institute of International Rivers And Eco-Security,Yunnan University,Kunming 650000,China;School of Ecology and Environmental Science,Yunan University,Kunming 650000,China;Yunnan Academy of Science and Technology Development,Kunming 650000,China)

机构地区：[1]云南大学国际河流与生态安全研究院,昆明650000 [2]云南大学生态与环境学院,昆明650000 [3]云南省科学技术发展研究院,昆明650000

出　　处：《环境工程》2022年第1期13-20,共8页Environmental Engineering

基　　金：高原湖泊水环境治理与功能提升技术研究与集成及应用示范(2018BC001)。

摘　　要：为了解复合人工湿地系统对低污染水中总氮的处理效果及微生物群落结构特征,以表流-潜流-沉水植物塘构建的复合人工湿地系统为研究对象,研究该系统对低污染水中TN、NH_(4)^(+)-N、NO_(3)^(-)-N、NO_(2)^(-)-N的去除效果,并采用高通量测序揭示各级单元微生物群落结构与差异性。结果表明:复合人工湿地系统在0.12-0.24 m^(3)/(m^(2)·d)的水力负荷下出水达到GB 3838—2002《地表水环境质量标准》Ⅳ类水质,对污染物均有较好的去除效果,对TN、NH_(4)^(+)-N的去除率为62.35%-76.86%、36.94%-74.68%。各级单元主要的脱氮微生物表现出较大差异,属水平上,表流湿地主要脱氮菌群为硝化螺旋菌属、996-1属和生丝菌属;潜流湿地主要脱氮菌群为贪铜菌属和不动杆菌属;沉水植物塘主要脱氮菌群为甲基娇养杆菌属和嗜甲基菌属。说明各单元对总氮的去除效果均有贡献且存在差异。To understand the treatment effect of total nitrogen in low-polluted wastewater by composite constructed wetland system and the characteristics of microbial community structure in the constructed wetland consisted of surface flow-subsurface flow-submerged plant pond,we researched the removal rates of TN,NH_(4)^(+)-N、NO_(3)^(-)-N and NO_(2)^(-)-N in the wastewater by the system.High throughput sequencing method was applied to reveal the microbial community structure and diversity in the units at all levels.The results showed that the effluent of the composite constructed wetland system reached the classⅣof Environmental Quality Standard for Surface Water under the hydraulic load of 0.12-0.24 m^(3)/(m^(2)·d),and had a good removal effect on pollutants.And the removal rate of TN,NH_(4)^(+)-N was 62.35%-76.86%and 36.94%-74.68%.The main denitrification microorganisms in each unit showed great differences.At the genus level,the main denitrification microorganisms in the surface flow wetland were Nitrospira,996-1 and Hyphomicrobium.The main denitrification bacteria groups in the subsurface flow wetland were Cupriavidus and Acinetobacter.The main denitrification microorganisms in the submerged plant pond were Methylotenera and Methylophilus.The results indicated that all the units contributed to the removal of total nitrogen but there were differences between them.

关 键 词：总氮 微生物多样性 复合人工湿地 低污染水 

分 类 号：X703[环境科学与工程—环境工程]