苏姜猪NR6A1和VRTN基因多态性及其与生产性状的关联分析  

Association between polymorphism of NR6A1 and VRTN genes and production traits in Sujiang pigs

作　　者：徐盼[1] 刘林雨 仲德 何鑫鑫 罗阳琦文 马政 XU Pan;LIU Linyu;ZHONG De;HE Xinxin;LUO Yangqiwen;MA Zheng(School of Animal Science and Technology,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;College of Animal Science and Technology,Jinling Institute of Technology,Nanjing 210038,China)

机构地区：[1]江苏农牧科技职业学院动物科技学院,江苏泰州225300 [2]金陵科技学院动物科学与技术学院,江苏南京210038

出　　处：《畜牧与兽医》2022年第4期10-16,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31702089);江苏高校“青蓝工程”;江苏省大学生创新创业训练计划项目(202012806026Y);江苏农牧科技职业学院自然科学基金(NSF200701,NSFPT201802)。

摘　　要：旨在探讨生殖细胞核因子(nuclear receptor subfamily 6 group A member 1,NR6A1)和椎体发育同源物(vertebrae development homolog, VRTN)基因的多态性对苏姜猪体重、体长、体高、胸围、胸宽、臀宽和背膘厚7个生产性状的影响。试验利用PCR扩增和酶切片段多态性技术检测苏姜猪及其亲本猪品种中NR6A1和VRTN基因的多态性,并将NR6A1和VRTN基因的多态性与苏姜猪的生产性状进行关联性分析。遗传多态性分析结果表明,NR6A1基因p.Pro192Leu (g.299084751 C>T)位点在姜曲海猪、枫泾猪和杜洛克猪群体中已经纯合,分别为CC、CC和TT基因型;在苏姜猪群体中有TT、TC和CC 3种基因型,基因型频率分别为0.397、0.482和0.121,基因型分布频率符合哈迪温伯格平衡(Hardy-Weinberg equilibrium, HWE,P>0.05),处于中度多态(0.250.05),VRTN基因对苏姜猪胸围、胸宽和臀宽均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。合并基因型对苏姜猪体长、胸围和背膘厚均有显著影响(P<0.05),对其他性状无显著影响(P>0.05)。综上可知,NR6A1和VRTN基因对苏姜猪的生产性状具有一定影响,为后续应用NR6A1和VRTN基因辅助选育苏姜猪提供参考。The study was aimed to explore the effects of NR6 A1 and VRTN gene polymorphism on the seven production traits in Sujiang pigs, including body weight, body length, body height, chest circumference, chest width, hip width and backfat thickness. In the experiment, PCR amplification and restriction endonuclease rection techniques were used to detect the polymorphism of NR6 A1 and VRTN genes in Sujiang pigs and their parent breeds, and the association between the polymorphism of NR6 A1 and VRTN genes and the production traits of Sujiang pigs was analyzed. The results of the genetic polymorphism analysis showed that the p.Pro192 Leu(g.299084751 C>T) locus of the NR6 A1 gene was homozygous in Jiangquhai pig, Fengjing pig and Duroc pig populations, which was CC, CC and TT genotype, respectively. There were three genotypes(TT, TC and CC) at the p.Pro192 Leu locus in the Sujiang pig population, and the genotype frequencies were 0.397, 0.482 and 0.121, respectively. The genotype distribution frequency was in accordance with the Hardy Weinberg equilibrium(HWE,P>0. 05) and was of moderate polymorphism( 0. 250. 05). The VRTN gene had significant effects on the chest circumference,chest width and hip width of the Sujiang pigs( P<0. 05),but had no significant effect on the other traits( P>0. 05). The combination genetypes of the NR6A1 and VRTN gene had significant effects on the body length,chest circumference and backfat thickness of the Sujiang pigs( P<0. 05),but had no significant effect on the other traits( P>0. 05). The NR6A1 and VRTN genes had certain effects on the production traits of the Sujiang pigs,which provided a reference for subsequent application of NR6A1 and VRTN genes to assist in breeding Sujiang pigs.

关 键 词：苏姜猪 生殖细胞核因子基因 椎体发育同源物基因 多态性 生产性状 

分 类 号：S813.3[农业科学—畜牧学]