Photocrosslinkable Col/PCL/Mg composite membrane providing spatiotemporal maintenance and positive osteogenetic effects during guided bone regeneration  

作　　者：Feilong Wang Dandan Xia Siyi Wang Ranli Gu Fan Yang Xiao Zhao Xuenan Liu Yuan Zhu Hao Liu Yongxiang Xu Yunsong Liu Yongsheng Zhou 

机构地区：[1]Department of Prosthodontics,Peking University School and Hospital of Stomatology,Beijing,100081,China [2]National Engineering Laboratory for Digital and Material Technology of Stomatology,National Clinical Research Center for Oral Diseases,National Center of Stomatology,Beijing Key Laboratory of Digital Stomatology,Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health,NMPA Key Laboratory for Dental Materials,Beijing,100081,China [3]Department of Dental Materials,Peking University School and Hospital of Stomatology,Beijing,100081,China [4]Central Laboratory,Peking University School and Hospital of Stomatology,Beijing,100081,China

出　　处：《Bioactive Materials》2022年第7期53-63,共11页生物活性材料（英文）

基　　金：This study was supported by the Innovation research program[HHKT-00-03];the National Natural Science Foundation of China[grant numbers 82170929,81970908,51901003,81200814,and 81771039].

摘　　要：Guided bone regeneration membranes have been effectively applied in oral implantology to repair bone defects.However,typical resorbable membranes composed of collagen(Col)have insufficient mechanical properties and high degradation rate,while non-resorbable membranes need secondary surgery.Herein,we designed a photocrosslinkable collagen/polycaprolactone methacryloyl/magnesium(Col/PCLMA/Mg)composite membrane that provided spatiotemporal support effect after photocrosslinking.Magnesium particles were added to the PCLMA solution and Col/PCLMA and Col/PCLMA/Mg membranes were developed;Col membranes and PCL membranes were used as controls.After photocrosslinking,an interpenetrating polymer network was observed by scanning electron microscopy(SEM)in Col/PCL and Col/PCL/Mg membranes.The elastic modulus,swelling behavior,cytotoxicity,cell attachment,and cell proliferation of the membranes were evaluated.Degradation behavior in vivo and in vitro was monitored according to mass change and by SEM.The membranes were implanted into calvarial bone defects of rats for 8 weeks.The Col/PCL and Col/PCL/Mg membranes displayed much higher elastic modulus(p<0.05),and a lower swelling rate(p<0.05),than Col membranes,and there were no differences in cell biocompatibility among groups(p>0.05).The Col/PCL and Col/PCL/Mg membranes had lower degradation rates than the Col membranes,both in vivo and in vitro(p<0.05).The Col/PCL/Mg groups showed enhanced osteogenic capability compared with the Col groups at week 8(p<0.05).The Col/PCL/Mg composite membrane represents a new strategy to display space maintenance and enhance osteogenic potential,which meets clinical needs.

关 键 词：Guided bone regeneration Magnesium PHOTOCROSSLINKING Polycaprolactone methacryloyl COLLAGEN 

分 类 号：R318[医药卫生—生物医学工程]