The protein corona modulates the inflammation inhibition by cationic nanoparticles via cell-free DNA scavenging  

作　　者：Xingliang Liu Huiyi Liang Yanzi Yan Jingjiao Wu Massimo Bottini Lixin Liu Yongming Chen 

机构地区：[1]School of Materials Science and Engineering,Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education,Sun Yat-sen University,Guangzhou,510275,China [2]Department of Experimental Medicine,University of Rome Tor Vergata,Rome,00133,Italy

出　　处：《Bioactive Materials》2022年第7期249-259,共11页生物活性材料（英文）

基　　金：The financial support from the National Natural Science Foundation of China(21875290);the key Areas Research and Development Program of Guangzhou(202007020006);support of Sun Yat-sen University(19lgjc01)was acknowledged for support。

摘　　要：A central paradigm in nanomedicine is that when synthetic nanoparticles(NPs)enter the body,they are immediately cloaked by a corona of macromolecules(mostly proteins)that mediates the role of the physico-chemical properties in the NP biological functions(the“coronation paradigm”).In this work,we focused on the assessment of the“coronation paradigm”for cationic NPs(cNPs)used as rheumatoid arthritis(RA)drugs due to their ability to scavenge cell-free DNA(cfDNA).We fabricated series of cNPs uniformly coated with single or di-hydroxyl groups and different types of amino groups and showed that hydroxylated nanoparticles displayed a prolonged retention in inflamed joints and greater anti-inflammatory effect in collagen-induced arthritis(CIA)rats than the non-hydroxylated analogues.Especially,the cNPs with secondary amines and a di-hydroxyl shell showed the best performance among the tested cNPs.Proteomic analysis showed that the cNPs with a di-hydroxyl shell adsorbed less opsonin proteins than the cNPs carrying mono hydroxyl groups and non-hydroxylated ones,which may provide a mechanistic explanation for the different biodistribution profiles of cNPs.Thus,this study suggests that the protein corona mediates the effects of the surface chemistry on the fate and functions of cNPs as anti-RA drugs.

关 键 词：Cationic nanoparticles Cell-free DNA Inflammation inhibition Protein corona Rheumatoid arthritis 

分 类 号：R364.5[医药卫生—病理学]