Cu(Ⅱ)持续负荷对A/O工艺中硝化菌种群结构及功能代谢的动态影响  

Dynamic effects of continuous Cu(Ⅱ) loading on the structure and functional metabolism of nitrifying bacteria in A/O process

作　　者：欧阳帆[1,2] 季民[2] 翟洪艳[2] 钟润生[1] 肖峰[3] OUYANG Fan;JI Min;ZHAI Hongyan;ZHONG Runsheng;XIAO Feng(School of Traffic and Environment,Shenzhen Institute of Information Technology,Shenzhen 518172,China;School of Environmental Science and Engineering,Tianjin University,Tianjin 300072,China;School of Renewable Energy,North China Electric Power University,Beijing 102206,China)

机构地区：[1]深圳信息职业技术学院交通与环境学院,深圳518172 [2]天津大学环境科学与工程学院,天津300072 [3]华北电力大学可再生能源学院,北京102206

出　　处：《环境工程学报》2022年第1期320-331,共12页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51178302);深圳市科技计划项目(JCYJ20180307155011964);深圳信息职业技术学院深圳市龙岗区校企合作项目(SZIIT2020KJ009);广东省普通高校重点领域专项(科技服务乡村振兴)项目(2021ZDZX4106)。

摘　　要：为探讨铜离子(Cu^(2+))对A/O反应器中硝化细菌的毒性机制,研究了Cu^(2+)持续负荷下A/O反应器中硝化细菌的基质代谢能力、呼吸速率(SOUR)、功能基因(amoA和nxrB)的表达和菌落结构变化情况。结果表明,呼吸速率和基因转录对Cu^(2+)毒性的响应较基质代谢更为敏感。主成分回归模型分析结果显示,活性污泥表面吸附的Cu^(2+)和活性污泥内部的Cu^(2+)对硝化细菌的呼吸抑制起主要作用。饱和型生物毒性模型分析结果显示,活性污泥内部的Cu^(2+)对氨氧化细菌(AOB)和亚硝酸盐氧化细菌(NOB)呼吸速率的半反应质量浓度分别为12.91 mg·L^(−1)和7.3 mg·L^(−1),内部Cu^(2+)的抑制效果最强。全细菌中的Bacteroidetes门和Firmicutes门对Cu^(2+)的耐受性最强,Nitrosomonas和Nitrospira是主要的硝化菌,并且优势OTU的菌群相对丰度与硝化功能基因的表达量表现出相同的变化规律。In order to discuss the toxic mechanism of bivalent copper ion(Cu^(2+))to nitrifying bacteria in A/O reactor,the matrix metabolic capacity,respiration rates(SOUR),expression of functional genes(amoA and nxrB)and community structures of nitrifying bacteria in A/O reactor under continuous Cu^(2+)loading were simultaneously studied.The results showed that respiration rate and gene transcriptional responses were more sensitive to Cu^(2+)toxicity than matrix metabolism.A principal component regression analysis model revealed that the surface adsorbed Cu^(2+)and the intracellular Cu^(2+)of the activated sludge played main roles in the inhibition of the respiration rates of nitrifying bacteria.A biological receptor model suggested that the half inhibitory concentrations of intracellular Cu^(2+)to ammonia oxidizing bacteria(AOB)and nitrite oxidizing bacteria(NOB)were 12.91 mg·L^(−1) and 7.3 mg·L^(−1),respectively.Bacteroidetes and Firmicutes at the phylum level had the strongest resistance to Cu^(2+)in the overall microbial community.Nitrosomonas and Nitrospira were the main nitrifying bacteria,and the relative abundance of the dominant nitrifying OTU showed the same change rule as the expression of nitrifying functional genes.

关 键 词：A/O工艺 Cu^(2+)毒性 硝化细菌 分子生物学 

分 类 号：X172[环境科学与工程—环境科学]