Challenges in Analysis of Hydrophilic Metabolites Using Chromatography Coupled with Mass Spectrometry  

作　　者：Qingyu Hu Huiru Tang Yulan Wang 

机构地区：[1]CAS Key Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Centre for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences(CAS),Wuhan 430071,People’s Republic of China [2]University of Chinese Academy of Sciences,Beijing 10049,People’s Republic of China [3]State Key Laboratory of Genetic Engineering,Zhongshan Hospital and School of Life Sciences,Laboratory of Metabonomics and Systems Biology,Human Phenome Institute,Fudan University,Shanghai 200433,People’s Republic of China [4]Singapore Phenome Center,Lee Kong Chian School of Medicine,Nanyang Technological University,Singapore 639798,Singapore

出　　处：《Journal of Analysis and Testing》2020年第3期140-162,共23页分析检测（英文）

基　　金：This work was supported by grant from the National Key R&D Program of China(2017YFC0906800).

摘　　要：Hydrophilic metabolites play important roles in cellular energy metabolism,signal transduction,immunity.However,there are challenges in both identification and quantification of the hydrophilic metabolites due to their weak interactions with C18-reversed-phase liquid chromatography(RPLC),leading to poor retention of hydrophilic metabolites on the columns.Many strategies have been put forward to increase the retention behavior of hydrophilic metabolites in the RPLC system.Non-derivatization methods are mainly focused on the development of new chromatographic techniques with different separation mechanisms,such as capillary electrophoresis,ion-pairing RPLC etc.Derivatization methods improve the hydrophobicity of metabolites and can enhance the MS response.This review mainly focused on the illustration of challenges of LCMS in the analysis of hydrophilic metabolomics field,and summarized the non-derivatization and derivatization strategies,with the intention of providing multiple choices for analysis of hydrophilic metabolites.

关 键 词：Hydrophilic metabolites Hydrophilic interaction chromatography Ion-pairing reversed-phase liquid chromatography Ion chromatography Capillary electrophoresis DERIVATIZATION 

分 类 号：O657.63[理学—分析化学]