UV Spectroscopic Method for Optimization and Determination of Glibenclamide in Bulk,Pharmaceutical Dosage Form and its Application for In Vitro Interaction Studies  

作　　者：Faiza Akhtar Somia Gul Salman Ashfaq Iqra Rehman Agha Zeeshan Mirza 

机构地区：[1]Department of Pharmaceutical Chemistry,Faculty of Pharmacy,Jinnah University for Women,Karachi 74600,Pakistan [2]Department of Pharmaceutical Chemistry,Faculty of Pharmacy,Ziauddin University,Karachi 75600,Pakistan [3]Department of Pharmaceutics,Faculty of Pharmacy,University of Karachi,Karachi 75270,Pakistan [4]Chemistry Department,Faculty of Applied Sciences,Umm Al-Qura University,Mecca 21955,Saudi Arabia

出　　处：《Journal of Analysis and Testing》2020年第4期281-290,共10页分析检测（英文）

摘　　要：A simple,easy and cost-efficient UV spectroscopic method was developed and validated for glibenclamide,the second generation of sulfonylureas,using 0.1 N NaOH as a solvent.The proposed method is linear(R^(2)>0.999)with the range 5-25μg mL^(−1),accurate(99.60%),precise(inter and intraday variation 0.241 and 0.019%,respectively)and robust(<1%).The quantification and detection limit were 1.46 and 0.48μg mL^(−1),respectively.The validated method was applied for in vitro interaction studies of glibenclamide(GLB)with commonly prescribed quinolones(ciprofloxacin,levofloxacin,moxifloxacin,and gemifloxacin)and nonsteroidal anti-inflammatory drugs(NSAIDs)(diclofenac sodium,ibuprofen,mefenamic acid,and aspirin)using UV spectrophotometer.The in vitro interaction studies were carried out in different artificially prepared physiological buffers at 37°C for 2 h.Results showed raised level of glibenclamide when interacted with gemifloxacin(pH 7.4),levofloxacin(pH 9),ciprofloxacin(pH 4.5)and with moxifloxacin(pH 4.5,7.4 and 9).Interaction with NSAIDs results in increased%availability of glibenclamide in the presence of diclofenac sodium,ibuprofen,and mefenamic acid at pH 4.5.The anticipated method can be successfully applied for the routine analysis and also for the interaction of glibenclamide with other drugs.

关 键 词：GLIBENCLAMIDE INTERACTION QUINOLONES NSAIDS UV spectroscopy 

分 类 号：O657.1[理学—分析化学]