Electrochemical Biosensor for Rapid Detection of Viable Bacteria and Antibiotic Screening  

作　　者：Nuvia Maria Saucedo Sira Srinives Ashok Mulchandani 

机构地区：[1]Department of Chemistry,University of California,Riverside,CA 92521,USA [2]Department of Chemical and Environmental Engineering,University of California,Riverside,CA 92521,USA [3]Present Address:Department of Chemistry,Southern Adventist University,Collegedale,TN 37315,USA [4]Present Address:Department of Chemical Engineering,Mahidol University,25/25 Puttamonthon 4 Rd.,10,Salaya,Nakhon Pathom 73170,Thailand

出　　处：《Journal of Analysis and Testing》2019年第1期117-122,共6页分析检测（英文）

摘　　要：We have developed a biosensor for the detection of live/viable bacteria based on the response of the conductive polymer 4-(3-pyrrolyl)butyric acid to glucose-induced metabolites.The polymer was electrochemically deposited and then functionalized with lectin through EDC/NHS chemistry to capture cells near the sensor surface and introduce selectivity for analytes.The addition of glucose to a three-electrode electrochemical cell containing 10 mM phosphate buffer and the bacteria-immobilized sensor produced an increase in the potential.When the bound bacteria were treated with antibiotics,the addition of glucose produced a notably reduced signal exhibiting the sensor’s potential to screen for the most effective antibiotic treatment.This biosensor having real-time responses,minimal sample preparation,and the ability to screen antibiotics demonstrates the speed,ease,and suitability essential for application in point-of-care services.The detection range was determined to be 6.0×10^(3)-9.2×10^(7)CFU/mL.

关 键 词：Point of care PATHOGEN Infectivity Antibiotic LECTIN POTENTIOMETRY 

分 类 号：TP2[自动化与计算机技术—检测技术与自动化装置]