Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis  

作　　者：Jian Li Bo Zheng Qian-Wen Zhang Yang Liu Cai-Feng Shi Feng-Bin Wang Kang Wang Xing-Hua Xia 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,China

出　　处：《Journal of Analysis and Testing》2017年第1期71-83,共13页分析检测（英文）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(21327902,21635004,21675079,21627806).

摘　　要：Attenuated total reflection surface-enhanced infrared absorption spectroscopy(ATR-SEIRAS)has recently been proven to be a powerful tool for bioanalysis.It enables in situ and in real-time observation of dynamic processes occurring on specific interface,revealing rich structural and functional information of biomolecules at sub monolayer level.The aim of this general review was to give an overview of the cutting edge applications of ATRSEIRAS.We start with description of the basic configuration of the standard ATR-SEIRAS platform.The enhanced mechanisms and methods to fabricate enhanced substrates are then presented.We discuss the recent developments,challenges and applications of ATR-SEIRAS in bioanalysis,mainly focusing on DNA analysis,protein behavior and cell properties.Finally,further development of the ATRSEIRAS technique with enhanced sensitivity,improved time and spatial resolutions will be prospected.

关 键 词：Surface-enhanced infrared absorption spectroscopy ATR-SEIRAS BIOANALYSIS DNA PROTEIN CELL 

分 类 号：TG1[金属学及工艺—金属学]