微波辐射预处理菌源对生物暗发酵制氢的影响  

Effect of microwave irradiation pretreatment of microbial sources on the dark fermentative hydrogen production

作　　者：张靖楠 昌行行 张嘉祺 何培新 张志平 宋丽丽 杨旭 魏涛 ZHANG Jingnan;CHANG Hanghang;ZHANG Jiaqi;HE Peixin;ZHANG Zhiping;SONG Lili;YANG Xu;WEI Tao(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]郑州轻工业大学食品与生物工程学院,河南郑州450001

出　　处：《轻工学报》2022年第2期30-37,共8页Journal of Light Industry

基　　金：国家自然科学基金项目(21706244);河南省科技攻关项目(182102210162,212102310077,212102110310)。

摘　　要：以牛粪堆肥为天然菌源、葡萄糖为发酵底物,考查微波辐射预处理菌源对生物暗发酵制氢的影响,并采用Illumina NovaSeq高通量测序技术分析微波辐射预处理前后菌源中细菌和古生菌的群落结构变化,揭示预处理引起的菌源微生物群落结构变化对暗发酵制氢的影响机制。结果表明,与对照组相比,微波辐射预处理后菌源的累积产H_(2)量和丁酸含量分别提高了27.9%和24.1%,菌源中微生物多样性下降,主要产氢微生物芽孢杆菌的相对丰度增加,产甲烷耗氢菌的相对丰度减小;产氢微生物的富集及耗氢菌活性的抑制可有效提高暗发酵制氢系统的产H_(2)量。With cow dung compost as the natural microflora and glucose as fermentation substrate,the effect of microwave irradiation pretreatment on dark fermentative hydrogen production was investigated.The microbial community structure of bacteria and archaea was analyzed by Illumina NovaSeq sequencing to reveal the effect mechanism of microwave irradiation pretreatment on bio-hydrogen production.The results showed that the hydrogen yield and content of butyrate were significantly increased after microwave irradiation pretreatment,which were 27.9%and 24.1%higher than that of the control.The diversity of microbial communities was reduced with microwave irradiation pretreatment,and the species abundance of the main hydrogen producing microorganisms of Bacillus spp.was increased,while the hydrogen consumption archaea of methanogens was decreased.The hydrogen production could be significantly improved with the enrichment of hydrogen producing microorganisms and suppression of the activity of hydrogen consuming microorganisms.

关 键 词：生物制氢 微波辐射预处理 牛粪堆肥 芽孢杆菌 产甲烷古菌 

分 类 号：TS01[轻工技术与工程]