基于纳米金/石墨烯修饰电极的免疫传感器检测噻虫啉  

Immunosensor based on gold nanoparticles/graphene modified electrode for thiacloprid detection

作　　者：姚军 毛慧 张芹[4] 赵国政 张晋华[1] Yao Jun;Mao Hui;Zhang Qin;Zhao Guozheng;Zhang Jinhua(School of Environmental and Biological Engineering,Nanjing University of Science and Technology University,Nanjing 210094,China;Taizhou Ecological Environment Bureau,Taizhou 225300,China;Taizhou Environmental Monitoring Center of Jiangsu Province,Taizhou 225300,China;School of Chemistry and Materials Science,Nanjing Normal University,Nanjing 210023,China;Key Laboratory of Magnetic Molecules and Magnetic Information Materials,Ministry of Education,Shanxi Normal University,Linfen 041004,China)

机构地区：[1]南京理工大学环境与生物工程学院,江苏南京210094 [2]泰州市生态环境局,江苏泰州225300 [3]江苏省泰州环境监测中心,江苏泰州225300 [4]南京师范大学化学与材料科学学院,江苏南京210023 [5]山西师范大学磁性分子与磁信息材料教育部重点实验室,山西临汾041004

出　　处：《南京理工大学学报》2022年第2期239-244,共6页Journal of Nanjing University of Science and Technology

基　　金：山西省高等学校科技成果转化培育项目(2020CG032);临汾市重点研发计划(社会发展领域)项目(1909)。

摘　　要：该文研制了纳米金/石墨烯/噻虫啉电化学免疫传感器。运用循环伏安法和电化学交流阻抗谱法对修饰过程中电极表面的电化学性质进行了研究。通过间接竞争法检测了杀虫剂噻虫啉,最低检出限为0.7 ng·mL^(-1),线性范围为10~5000 ng·mL^(-1)。经检测纳米金/石墨烯/噻虫啉电化学免疫传感器具有良好的重复性与稳定性,并对香蕉、西红柿、苹果和大米实际样品进行测定,回收率范围87.1%~113.4%。An electrochemical immunosensor based on gold nanoparticles/graphene modified electrode was developed in this paper.The electrochemical properties of the modified electrode were studied by cyclic voltammetry and electrochemical impedance spectroscopy.Insecticide thiacloprid was detected by indirect competitive method.The lowest detection limit was 0.7 ng·mL^(-1),and the linear range was 10~5000 ng·mL^(-1).The gold nanoparticles/graphene/thiacloprid immunosensor has good repeatability and stability.The samples of banana,tomato,apple and rice were determined,and the recoveries ranged from 87.1%to 113.4%.

关 键 词：纳米金 石墨烯 免疫传感器 噻虫啉抗体 噻虫啉 

分 类 号：TS202.3[轻工技术与工程—食品科学]