铁调节蛋白2高表达对MPP^(+)诱导的PC12细胞损伤的影响  

EFFECT OF THE HIGH EXPRESSION OF IRON REGULATORY PROTEIN 2 ON 1-METHYL-4-PHENYLPYRIDINIUM-INDUCED PC12 CELL INJURY

作　　者：姚征洋 贾凤菊[1] 傅琳 焦倩[1] 陈曦[1] 杜希恂[1] 姜宏[1] YAO Zhengyang;JIA Fengju;FU Lin;JIAO Qian;CHEN Xi;DU Xixun;JIANG Hong(School of Basic Medicine, Qingdao University,Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines, Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao 266071, China)

机构地区：[1]青岛大学基础医学院,山东省神经相关疾病的机制与重点防治实验室,山东省沿海地区神经退变疾病协同创新中心,山东青岛266071

出　　处：《精准医学杂志》2022年第2期95-98,104,共5页Journal of Precision Medicine

基　　金：国家自然科学基金面上项目(32171131);山东省自然科学基金重大基础研究项目(ZR2019ZD31);山东省自然科学基金青年项目(ZR2020QC088)。

摘　　要：目的探讨铁调节蛋白2(IRP2)高表达对1-甲基-4-苯基吡啶阳离子(MPP^(+))损伤的PC12细胞存活率的影响。方法以MPP^(+)诱导PC12细胞损伤,依据对细胞的处理方式不同分为vector组(转染Myc-vector质粒)、IRP2组(转染Myc-IRP2质粒)、vector/MPP^(+)组(转染Myc-vector质粒并以MPP^(+)诱导细胞损伤)和IRP2/MPP^(+)组(转染Myc-IRP2质粒并以MPP^(+)诱导细胞损伤),采用免疫印迹法检测各组细胞中IRP2的表达水平,采用CCK-8测定法检测各组细胞的存活率。结果免疫印迹法检测结果显示,IRP2组和IRP2/MPP^(+)组细胞中IRP2的相对表达量较vector组和vector/MPP^(+)组均明显升高(F=15.38,P<0.05)。CCK-8法检测结果显示,IRP2和MPP^(+)可协同诱导PC12细胞损伤(F_(转染类型*损伤处理)=4.34,P<0.05);各组间细胞存活率比较差异均有显著性(P<0.05)。结论IRP2高表达和MPP^(+)均可致PC12细胞损伤,且IRP2高表达可加剧MPP^(+)对PC12细胞的损伤作用。Objective To investigate the effect of the high expression of iron regulatory protein 2(IRP2)on the viability of PC12 cells induced by 1-methyl-4-phenylpyridinium(MPP^(+)).Methods PC12 cell injury was induced by MPP^(+),and accor-ding to the treatment method,the PC12 cells were divided into vector group(transfected with Myc-vector plasmid),IRP2 group(transfected with Myc-IRP2 plasmid),vector/MPP^(+) group(transfected with Myc-vector plasmid and treated with MPP^(+)to induce cell injury),and IRP2/MPP^(+) group(transfected with Myc-IRP2 plasmid and treated with MPP^(+) to induce cell injury).Wes-tern blotting was used to measure the protein expression level of IRP2 in each group,and CCK-8 assay was used to measure cell viability in each group.Results Western blotting showed that the relative expression level of IRP2 in the IRP2 group and the IRP2/MPP^(+) group was significantly higher than that in the vector group and the vector/MPP^(+) group(F=15.38,P<0.05).CCK-8 assay showed that IRP2 and MPP^(+) had a synergistic effect on PC12 cell injury(F_(transfection*damage)=4.34,P<0.05),there was a significant difference in cell viability between these groups(P<0.05).Conclusion Both high IRP2 expression and MPP^(+) can induce PC12 cell injury,and high IRP2 expression can exacerbate the damaging effect of MPP^(+) on PC12 cells.

关 键 词：帕金森病 铁调节蛋白质2 1-甲基-4-苯基吡啶 阳离子 铁代谢 细胞存活 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R338.2[医药卫生—临床医学]