五倍子单宁酸中没食子酸的去除及其含量测定  

Removal of gallic acid impurities from Galla chinensis tannic acid and HPLC analysis

作　　者：李志遥 李珂 张妮[2,3] 任海[2,3] 宋俊蓉[2,3] 王绍江 陈超[2,3] 潘卫东 LI Zhi-yao;LI Ke;ZHANG Ni;REN Hai;SONG Jun-rong;WANG Shao-jiang;CHEN Chao;PAN Wei-dong(School of Pharmaceutical Sciences,Guizhou University,Guiyang 550002,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences/Guizhou Provincial Engineering Research Center for Natural Drugs,Guiyang 550014,China;Gallotannin Co.,Ltd,Guiyang 550001,China)

机构地区：[1]贵州大学药学院,贵州贵阳550002 [2]贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [3]贵州省中国科学院天然产物化学重点实验室贵州省天然药物工程研究中心,贵州贵阳550014 [4]贵阳单宁科技有限公司,贵州贵阳550001

出　　处：《广西大学学报（自然科学版）》2022年第1期229-234,共6页Journal of Guangxi University（Natural Science Edition）

基　　金：贵州省科技计划项目(黔科合支撑〔2019〕2786号);贵州省高层次创新型人才项目(黔科合平台人才〔2016〕4037);贵州省科技计划项目(黔科合支撑〔2020〕4Y157号)。

摘　　要：单宁酸是五倍子提取物中的主要成分,被广泛应用于医药、食品、日化等方面。但由常规方法制备的单宁酸中常含有没食子酸杂质,影响产品的品质和应用。为了提高五倍子单宁酸提取物的纯度,以单宁酸为原料、没食子酸含量为考察指标,采用简单易得的无机碱(碳酸氢钠)选择性去除没食子酸,对单宁酸的提纯工艺进行优化。对优化后的工艺进行放大实验,并考察该方法的适用性。结果表明:纯化前单宁酸中没食子酸质量分数为2.47%,纯化后没食子酸质量分数为0.15%,且该工艺放大至160 kg的规模下仍能够实现重复。本纯化工艺操作简便、经济性好,能够实现高纯度单宁酸的高效制备,具有显著的应用前景和实用价值。Tannic acid is one of the main constituents in the Galla Chinensis,which has widely used in medicine,food,daily chemical,and other aspects.However,tannic acid prepared by conventional methods often contains gallic acid impurities,which affects its quality and application.To improve the purity of tannic acid,the corresponding purification process was optimized to reduce the content of gallic acid in tannic acid by using the inorganic base NaHCO_(3).The applicability of the method was further investigated by the scale-up experiment under optimized condition.As a result,the content of gallic acid in tannic acid was reduced from 2.47% to 0.15% after purification.Moreover,the process can be repeated when scaled up to 160 kg.The purification process is simple,economical,and can realize the efficient preparation of high-purity tannic acid,which has a significant application prospect and practical value.

关 键 词：单宁酸 没食子酸 精制 高效液相色谱方法 纯化工艺 

分 类 号：TQ941[化学工程]