急性T淋巴细胞白血病的miR-211、miR-155、C-myc表达水平及相关性研究  

Expression Level and Correlation of miR-211, miR-155, C-myc in Acute T Lymphocytic Leukemia

作　　者：郑阳[1] 陈良[2] 郭树霞[3] 宋银森 ZHENG Yang;CHEN Liang;GUO Shu-Xia;SONG Yin-Sen(Depariment of Translational Medicine Research Center,People's Hospital of Zhengzhou,Zhengzhou 450000,Henan Province,China;Depariment of Laboratory,People's Hospital of Zhengzhou,Zhengzhou 450000,Henan Province,China;Department of Blood Specialty,People's Hospital of Zhengzhou,Zhengzhou 450000,Henan Province,China)

机构地区：[1]郑州人民医院转化医学研究中心,河南郑州450000 [2]郑州人民医院检验科,河南郑州450000 [3]郑州人民医院血液科,河南郑州450000

出　　处：《中国实验血液学杂志》2022年第2期425-429,共5页Journal of Experimental Hematology

摘　　要：目的:分析并探讨急性T淋巴细胞白血病(T-ALL)的miR-211、miR-155、C-myc表达及相关性,旨在为早期诊断及预后监测提供依据。方法:选择2014年6月-2017年6月于郑州人民医院接受诊治的T-ALL患者96例,另选同期体检健康的志愿者69例为对照组,采用实时荧光定量PCR测定各组外周血单个核细胞中miR-211、miR-155、C-myc表达水平,通过Kaplan-Meier分析T-ALL患者生存情况及miR-211、miR-155和C-myc与预后的关系,Pearson相关分析评价miR-211、miR-155和C-myc与疾病危险度的相关性。结果:T-ALL组miR-211 mRNA、miR-155 mRNA、C-myc mRNA表达水平均明显高于对照组(P<0.01),未缓解组均明显高于缓解组(P<0.01),高危组亦明显高于低危组和中危组(P<0.01)。miR-211低表达患者生存时间明显长于高表达患者(P<0.01),miR-155低表达患者生存时间明显长于高表达患者(P<0.01),C-myc低表达患者生存时间亦明显长于高表达患者(P<0.01)。miR-211、miR-155和C-myc高表达与疾病高危呈线性正相关(r=0.749、0.781、0.804)。结论:T-ALL患者miR-211、miR-155、C-myc表达上调,其表达水平与预后密切相关,且与疾病危险度呈线性正相关。Objective: To investigate the expression and correlation of miR-211, miR-155, and C-myc in acute T lymphocytic leukemia(T-ALL), aiming to provide evidence for the diagnosis and treatment. Methods: A total of 96 T-ALL patients who were diagnosed and treated in People′s Hospital of Zhengzhou from June 2014 to June 2017 were selected, and 69 healthy volunteers who had a physical examination were selected as control group in the same period. Real-time fluorescent quantitative PCR(RT-PCR) was used to determine the expression levels of miR-211, miR-155, and C-myc in peripheral blood mononuclear cells in each group. Kaplan-Meier was used to analyze the survival of T-ALL patients and correlation of miR-211, miR-155, and C-myc with prognosis. Pearson correlation analysis was used to evaluate the correlation of miR-211, miR-155, and C-myc with disease risk. Results: The expression levels of miR-211 mRNA, miR-155 mRNA, and C-myc mRNA in T-ALL group were higher than those in the control group(P<0.01), those in non-remission group were higher than those in remission group(P<0.01), and those in high-risk group were also higher than those in low-risk group and intermediate-risk group(P<0.01). The survival time of T-ALL patients with low miR-211 expression was longer than that with high miR-211 expression(P<0.01), that with low miR-155 expression was longer than that with high miR-155 expression(P<0.01), and that with low C-myc expression was also longer than that with high C-myc expression(P<0.01). The high expression of miR-211, miR-155, and C-myc was linearly positively correlated with high risk of disease(r=0.749, 0.781, 0.804). Conclusion: The expressions of miR-211, miR-155, and C-myc are up-regulated in T-ALL patients, closely related to prognosis, and linearly positively correlated with disease risk.

关 键 词：急性T淋巴细胞白血病 miR-211 MIR-155 C-MYC 相关性 

分 类 号：R733.71[医药卫生—肿瘤]