急性髓系白血病细胞对骨髓基质细胞增殖、凋亡的影响  

Effect of Acute Myeloid Leukemia Cells on the Proliferation and Apoptosis of Bone Marrow-Derived Mesenchymal Stromal Cells

作　　者：陈泽慧 郑雅心 司君齐 袁田 张志蓉[2] 田晨 CHEN Ze-Hui;ZHENG Ya-Xin;SI Jun-Qi;YUAN Tian;ZHANG Zhi-Rong;TIAN Chen(Department of Hematology,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy Tianjin,Tianjin's Clinical Research Center for Cancer,Tianjin 300060,China;Depariment of Oncology,Hetian District People's Hospital,Hetian 848000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]天津医科大学肿瘤医院血液科,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,天津市恶性肿瘤临床医学研究中心,天津300060 [2]新疆和田地区人民医院肿瘤科,新疆和田848000

出　　处：《中国实验血液学杂志》2022年第2期430-434,共5页Journal of Experimental Hematology

基　　金：天津市卫生健康科技项目(ZC20171);国家自然科学基金(81800148);新疆维吾尔自治区自然科学基金面上项目(2022D01A09)。

摘　　要：目的:研究在白血病微环境下,急性髓系白血病细胞对骨髓基质细胞(BM-MSC)增殖、凋亡的影响。方法:选取过表达MLL-AF9的急性髓系白血病(AML)小鼠模型与野生型(WT)小鼠为研究对象。应用流式细胞分选仪分选WT小鼠与AML小鼠来源的BM-MSC并计数。通过倒置荧光显微镜分析不同来源的BM-MSC的形态及生长差异性。采用Brdu法检测不同来源的BM-MSC增殖率。采用Annexin V/PI法检测不同来源的BM-MSC凋亡率。结果:与WT小鼠来源的BM-MSC比较,AML来源的BM-MSC数量显著增加(P<0.01),细胞增殖率显著增高(P<0.001);凋亡率显著降低(P<0.05)。在体外培养BM-MSC,加入条件培养基的BM-MSC生长速度显著加快,但形态没有明显差异。结论:在白血病微环境中,AML细胞能够促进BM-MSC增殖,抑制其凋亡。Objective: To investigate the effect of acute myeloid leukemia cells in leukemia-microenvironment on proliferation and apoptosis of bone marrow-derived mesenchymal stromal cells(BM-MSC). Methods: Acute myeloid leukemia(AML) murine models overexpressing MLL-AF9 were established. The number of BM-MSC of wild type(WT) and AML-derived mice were analyzed by flow cytometry. Morphology and growth differences between WT and AML-derived BM-MSC were analyzed by inverted fluorescence microscope. Proliferation and apoptosis of BM-MSC between these two groups were detected by Brdu and Annexin V/PI. Results: Compared with WT-derived BM-MSC, the number and proliferation rate of AML-derived BM-MSC significantly increased(P<0.01, P<0.001), while apoptosis rate decreased(P<0.05). When cultured in vitro, BM-MSC grew faster under conditional medium. Conclusion: AML cells can promote proliferation and inhibit apoptosis of BM-MSC.

关 键 词：急性髓系白血病 骨髓基质细胞 增殖 凋亡 小鼠 

分 类 号：R733.71[医药卫生—肿瘤]