厌氧氨氧化污泥胞外多糖的提取方法比较研究  

Comparative Study on the Extraction Methods of Extracellular Polysaccharides Substances from Anammox Sludge

作　　者：王雯谚 王兰 王茹 邓良伟[4,5] WANG Wenyan;WANG Lan;WANG Ru;DENG Liangwei(School of Environment and Municipal Engineering,Xi’an University of Architecture and Technology,Xi'an 710055,China;Shaanxi Provincial Key Laboratory of Environmental Engineering,Chengdu 610041,China;Key Laboratory of Northwest Water Resources and Environmental Ecology of Ministry of Education,Chengdu 610041,China;Biogas Institute of Ministry of Agriculture,Xi'an 710055,China;Laboratory of Development and Application of Rural Renewable Energy,Xi'an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055 [2]陕西省环境工程重点实验室,四川成都610041 [3]西北水资源与环境生态教育部重点实验室,四川成都610041 [4]农业农村部沼气科学研究所,陕西西安710055 [5]农业农村部农村可再生能源开发利用重点实验室,陕西西安710055

出　　处：《环境科学与技术》2021年第12期105-112,共8页Environmental Science & Technology

基　　金：国家自然科学基金项目(31972613,51808433);中央级公益性科研院所基本科研业务费专项(1610012020001_03202);四川省科技计划项目(2021YJ0319);国家现代农业产业技术体系专项资金(CARS-35);中国农业农村部,农村可再生能源开发与应用重点实验室开放课题(20172007);中国农业科学院科技创新工程(CAAS-ASTIP-2016-BIOMA)。

摘　　要：厌氧氨氧化细菌生长缓慢,颗粒化有助于其在反应器中的持留,防止污泥流失,而胞外多糖作为颗粒化的生物胶,是其颗粒化的重要因素,因此研究其胞外多糖的代谢途径及结构对厌氧氨氧化工艺的推广应用至关重要。但是,目前尽管厌氧氨氧化污泥的胞外聚合物的提取方法众多,但尚无针对其胞外多糖提取方法的比较研究。因此,该研究对比了3种胞外聚合物(EPS)的提取方法:甲酰胺-NaOH法、加热-Na_(2)CO_(3)法和甲醛-NaOH法。通过比较其污泥形态、EPS产量、TOC总量、3D-EEM图谱及污泥细胞裂解程度,发现甲醛-NaOH法所得到的EPS提取液胞外多糖含量最高、胞外蛋白含量最低、细胞裂解程度最低,是厌氧氨氧化颗粒污泥中胞外多糖的最佳提取方法。该研究可为厌氧氨氧化污泥胞外多糖的代谢途径和结构研究提供技术支撑。Anammox bacteria have long been known as its slow-growing.Granulation is benefit to prevent sludge loss by retaining microorganisms within the reactor,and polysaccharide as the biological gels determined the degree of granulation.So that,it is essential to explore the metabolic pathways and molecular structures of polysaccharides.Although there are many methods for the extraction of extracellular polymeric substances(EPS)from Anammox bacteria,but there still little reports about the comparative studies on the extraction of extracellular polysaccharides.Here we compared three EPS extraction methods,formamide with NaOH,heating-Na_(2)CO_(3)and formaldehyde with NaOH,and found that EPS obtained by formaldehyde with NaOH had the highest polysaccharide content,the lowest protein content and the lowest degree of cell lysis,by comparing the morphological characteristics of sludge,EPS yields,TOC contents,3D-EEMs fluorescence spectra and the degree of cell apoptosis.Therefore,formaldehyde with NaOH is the best approach to obtain the extracellular polysaccharides from Anammox bacteria and the study is helpful to understanding the metabolic pathways and molecular structures of extracellular polysaccharides.

关 键 词：厌氧氨氧化 胞外聚合物 胞外多糖 甲醛-NaOH法 甲酰胺-NaOH法 加热-Na_(2)CO_(3)法 

分 类 号：X703[环境科学与工程—环境工程]