沉默EGFL6基因表达抑制卵巢癌细胞增殖、迁移、侵袭和上皮间质转化  

Silencing of EGFL6 inhibits proliferation,migration,invasion,and epithelial-mesenchymal transition of ovarian cancer cells

作　　者：杨夏楠[1] 赵丹丹 司晗 杨平娟 樊冬梅[1] YANG Xianan;ZHAO DANDan;SI Han;YANG Pingjuan;Fan Dongmei(Department of Gynecology,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang71003,CHN)

机构地区：[1]河南科技大学第一附属医院,妇三科,河南洛阳471003

出　　处：《河南大学学报（医学版）》2022年第2期117-122,148,共7页Journal of Henan University:Medical Science

摘　　要：目的:探讨表皮生长因子样结构域蛋白6(EGFL6)在卵巢癌中的表达及其对卵巢癌细胞增殖、迁移、侵袭和上皮间质转化(Epithal-Mesenchymal Transition,EMT)的影响。方法:实验分为si-NC组和si-EGFL6组,将si-EGFL6使用Lipofectamine2000转染至卵巢癌SK-OV-3和OV-90细胞。使用在线工具GEPIA和KM-plotter分析EGFL6在卵巢癌中的表达水平及其与预后的关系。CCK-8实验检测SK-OV-3和OV-90细胞的增殖,划痕愈合实验和Transwell实验分别检测SK-OV-3和OV-90细胞的迁移和侵袭能力,q RT-PCR实验检测SK-OV-3和OV-90细胞中EGFL6的表达水平,Westernblot检测EGFL6及EMT相关基因E-cadherin、Vimentin和N-cadherin的蛋白表达水平。结果:EGFL6在卵巢癌高表达,和卵巢癌的不良预后相关,具有临床诊断意义。沉默EGFL6表达后,SK-OV-3和OV-90卵巢癌细胞增殖能力受到明显抑制(P<0.01),迁移和侵袭能力降低(P<0.01),EMT相关蛋白Vimentin和N-cadherin蛋白的表达降低,E-cadherin蛋白的表达升高。结论:沉默EGFL6能够抑制卵巢癌细胞增殖、迁移、侵袭和上皮间质转化。Objective:To explore the expression of epidermal growth factor-like domain protein 6(EGFL6) in ovarian cancer and its effects on the proliferation,migration,invasion and epithelial-mesenchymal transition(EMT) of ovarian cancer cells.Methods:The experiment was divided into si-NC group and si-EGFL6 group,and si-EGFL6 was transfected into ovarian cancer cells SK-OV-3 and OV-90 employing Lipofectamine 2000.The online tools GEPIA and KM-plotter were used to analyze the expression of EGFL6 in ovarian cancer and its relationship with prognosis.The CCK-8 assay was performed to detect the proliferation of SK-OV-3 and OV-90 cells,the scratch healing assay and the trans well assay were used to detect the migration and invasion ability of SK-OV-3 and OV-90 cells,respectively.The expression of EGFL6 was detected by qRT-PCR assay.Western blot wa.s used to detect the protein expression levels of EGFL6 and EMT-related genes E-cadherin,Vimentin and N-cadherin.Results:EGFL6 was highly expressed in ovarian cancer,and its expression was related to the poor prognosis of patients with ovarian cancer,indicating the diagnostic role of EGFL6 in ovarian cancer.Following si-EGFL6 transfection,the proliferation ability of SK-OV-3 and OV-90 cells was significantly inhibited(P<0.01),the migration and invasion capabilities of SK-OV-3 and OV-90 cells were reduced(P<0.01).Silencing of EGFL6 reduced the protein expression levels of Vimentin and N-cadherin,and increased the protein expression levels of E-cad her in in SK-OV-3 and OV-90 cells.Conclusion:Silencing of EGFL6 could inhibit the proliferation,migration,invasion and EMT of ovarian cancer cells.

关 键 词：EGFL6 卵巢癌 EMT 迁移 侵袭 

分 类 号：R361.3[医药卫生—病理学]