不同磷形态对水稻根系细菌群落特征的影响  

Effects of different phosphorus forms on the characteristics of bacterial community in rice roots

作　　者：王萌[1] 范分良[1] 易可可[1] 徐磊 徐壮 张佳音 孟莉 王赛 李旭 宋阿琳[1] WANG Meng;FAN Fen-liang;YI Ke-ke;XU Lei;XU Zhuang;ZHANG Jia-yin;MENG Li;WANG Sai;LI Xu;SONG A-lin(Institute of Agricultural Resources and Regional Planning of CAAS/Key Laboratory of Plant Nutrition and Fertilizer of Ministry of Agriculture,Beijing 100081;China Selenium Industry Research Institute,Ankang Shaanxi 725000)

机构地区：[1]中国农业科学院农业资源与农业区划研究所/农业农村部植物营养与肥料重点实验室,北京100081 [2]中国富硒产业研究院,陕西安康725000

出　　处：《中国土壤与肥料》2022年第3期173-181,共9页Soil and Fertilizer Sciences in China

基　　金：中央级公益性科研院所基本科研业务费专项(1610132019011,1610132020012)。

摘　　要：为了探索不同磷形态处理下土壤、水稻根际和根内环境中细菌群落组成、结构、多样性,从而挖掘不同磷形态下水稻根际中介导植物-微生物相互作用的细菌群落特征。以籼稻(kasalath)为试验材料,采集南京的低磷土壤进行为期8周的水稻盆栽试验,采用高通量测序方法(16S rDNA Illumina测序),并结合土壤有效磷浓度和水稻生物学指标,分别比较不同磷形态处理下水稻生长和根际土、根内细菌微生物群落结构组成和多样性。研究发现,有机磷和无机磷处理显著提高了土壤有效磷含量和磷酸酶活性,其中与不加磷对照相比,无机磷处理显著提高了植株全磷含量,有机磷处理与无机磷处理无显著性差异;在根际土和根内细菌中,有机磷和对照处理植株细菌多样性均显著高于无机磷处理;不同磷形态下水稻的根系细菌多样性和丰富度均显著(P<0.001)低于根际和非根际土壤细菌多样性和丰富度指数。不同处理中主要细菌包括:厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、Ignavibacteriae、绿弯菌门(Chloroflexi)。无机磷处理与对照、有机磷处理相比较,在根际土壤和根内细菌中微杆菌科(Microbacteriaceae)、链霉菌科(Streptomycetaceae)相对丰度较低,高温放线菌科(Thermoactinomycetaceae)、Ignavibacteriaceae显著高于对照和有机磷处理,其中芽孢杆菌科(Bacillaceae)在根际、非根际土壤及根系相对丰度均低于对照和有机磷处理。根际土细菌多样性与植株全磷呈极显著负相关;根系细菌多样性与地上部生物量呈极显著负相关(P<0.01),与有效磷和磷酸酶活性呈显著负相关(P<0.05);根系细菌丰富度与有效磷呈显著负相关:非根际土壤细菌多样性与有效磷呈极显著负相关,与磷酸酶活性和植株全磷呈显著负相关;非根际土壤细菌多样性和丰富度均与有效磷呈极显著负相关,与�In order to explore the composition,structure and diversity of bacterial communities in the soil,rice rhizosphere and root endophytic bacteria under different phosphorus forms,and to explore the characteristics of bacterial communities that mediate plant-microbe interactions in rice rhizosphere under different phosphorus forms,using indica rice(kasalath)as the experimental material,collecting low-phosphorus soil in Nanjing for an eight-week rice pot experiment,using highthroughput sequencing(16S rDNA Illumina sequencing),and combined with soil available phosphorus concentration and rice biological indicators,respectively,rice growth and rhizosphere and root endophytic bacterial microbial community structure and diversity under different phosphorus forms were compared.The study found that organic phosphorus and inorganic phosphorus treatments significantly increased soil available phosphorus content and phosphatase activity.Compared with the control without phosphorus,inorganic phosphorus treatment significantly increased the total phosphorus content of plants,while there was no significant difference between organic phosphorus treatment and inorganic phosphorus treatment.Among rhizosphere and rhizobacteria,the bacterial diversity of plants treated with organic phosphorus and without phosphorus treatment was significantly higher than that of inorganic phosphorus treatment.The bacterial diversity and richness of rice roots in different phosphorus treatments were both significantly(P<0.001)lower than the rhizosphere and non-rhizosphere soil bacterial diversity and richness index.The main bacteria in different treatments mainly include:Firmicutes,Proteobacteria,Actinobacteria,Acidobacteria,Ignavibacteriae,Chloroflexi.Compared with inorganic phosphorus treatment and no phosphorus and organic phosphorus treatment,the relative abundance of Microbacteriaceae and Streptomycetaceae in rhizosphere soil and root bacteria was lower,and Thermoactinomycetaceae,Ignavibacteriaceae were significantly higher than the treatments wit

关 键 词：磷形态 根际微生物群落 水稻 高通量测序 微生物多样性 

分 类 号：S154.3[农业科学—土壤学] S511[农业科学—农业基础科学]