过表达LncRNA SNHG7通过miR-342-3p/FOXM1轴促进胃癌细胞增殖、侵袭及其EMT  

Overexpression of lncRNA SNHG7 promotes the proliferation,invasion and EMT of gastric cancer cells through miR-342-3p/FOXM1 axis

作　　者：罗威[1] 潘曙光[1] 肖华[2] 唐卫[1] LUO Wei;PAN Shuguang;XIAO Hua;TANG Wei(Department of Colorectal Surgery,Hunan Cancer Hospital,Changsha 410013,China;Department of Hepatobiliary Surgery,Hunan Cancer Hospital,Changsha 410013,China)

机构地区：[1]湖南省肿瘤医院结直肠外科,湖南长沙410013 [2]湖南省肿瘤医院肝胆外科,湖南长沙410013

出　　处：《现代医学》2022年第1期1-9,共9页Modern Medical Journal

基　　金：湖南省卫生健康委科研计划项目(20200608)。

摘　　要：目的:探讨lncRNA SNHG7在胃癌MGC-803细胞中的表达及其对MGC-803细胞增殖、侵袭及上皮间质转化(epithelial-mesenchymal transition,EMT)的影响。方法:收集胃癌组织及其癌旁正常组织标本,培养胃癌细胞(MGC-803)和癌旁正常组织细胞(GES-1),采用Real-time PCR检测SNHG7、miR-342-3p、FOXM1在胃癌组织、癌旁正常组织、MGC-803细胞和GES-1细胞中的表达。通过MTT法、细胞侵袭和Western blot实验检测下调lncRNA SNHG7对MGC-803细胞增殖、侵袭及EMT的影响。通过miRcode软件和双荧光素酶报告基因活性检测方法剖析lncRNA SNHG7和miR-342-3p之间的关系;下调miR-342-3p,分析MGC-803细胞的发展趋势。同时上调lncRNA SNHG7和miR-342-3p,检测lncRNA SNHG7通过miR-342-3p对MGC-803细胞的增殖、侵袭及EMT的影响;TargetScan软件分析miR-342-3p与FOXM1的结合位点,双荧光素酶报告检测miR-342-3p与FOXM1之间的靶向关系。MTT法检测下调FOXM1对MGC-803细胞增殖的影响。细胞侵袭和Western blot分别检测下调FOXM1对MGC-803细胞侵袭和EMT的影响。结果:与GES-1细胞相比,MGC-80细胞中lncRNA SNHG7高表达(P<0.01),miR-342-3p低表达(P<0.01),FOXM1高表达(P<0.01)。与癌旁正常组织相比,胃癌组织中lncRNA SNHG7高表达(P<0.01),miR-342-3p低表达(P<0.01),FOXM1高表达(P<0.01)。下调lncRNA SNHG7抑制MGC-803细胞的增殖、侵袭及EMT。LncRNA SNHG7与miR-342-3p之间具有负调控及靶向关系。下调miR-342-3p促进MGC-803细胞的增殖、侵袭及EMT;上调lncRNA SNHG7通过miR-342-3p促进MGC-803细胞的增殖、侵袭及EMT。miR-342-3p与FOXM1之间具有负调控及靶向关系。下调FOXM1对MGC-803细胞的增殖、侵袭及EMT具有抑制作用。lncRNA SNHG7通过miR-342-3p上调FOXM1。结论:过表达LncRNA SNHG7通过miR-342-3p/FOXM1轴促进胃癌细胞增殖、侵袭及其EMT。Objective:To investigate the expression of lncRNA SNHG7 in gastric cancer MGC-803 cells and its effects on the proliferation,invasion and EMT of MGC-803 cells.Methods:Specimens of gastric cancer tissues and adjacent normal tissues were collected,gastric cancer cells(MGC-803)and adjacent normal tissue cells(GES-1)were cultured,real-time PCR was used to detect the expression of SNHG7,miR-342-3 p and FOXM1 in gastric cancer tissues,adjacent normal tissues,MGC-803 cells and GES-1 cells.The effects of down-regulation of lncRNA SNHG7 on proliferation,invasion and EMT of MGC-803 cells were detected by MTT assay,cell invasion and Western blot assay.The relationship between lncRNA SNHG7 and miR-342-3 p was analyzed using miRcode software and dual luciferase reporter gene activity detection method.MiR-342-3 p was down-regulated to analyze the development trend of MGC-803 cells,meanwhile,lncRNA SNHG7 and miR-342-3 p were up-regulated,the effects of lncRNA SNHG7 on the proliferation,invasion and EMT of MGC-803 cells via miR-342-3 p were detected.TargetScan software analyzed the binding sites between miR-342-3 p and FOXM1,dual luciferase reporting detected the targeting relationship between miR-342-3 p and FOXM1.MTT assay was used to detect the effect of down-regulated FOXM1 on MGC-803 cell proliferation.The effects of down-regulated FOXM1 on MGC-803 cell invasion and EMT were detected by cell invasion and Western blot respectively.Results:Compared with GES-1 cells,lncRNA SNHG7 was highly expressed in MGC-80 cells(P<0.01),miR-342-3 p was lowly expressed(P<0.01),and FOXM1 was highly expressed(P<0.01).Compared with normal tissues adjacent to cancer,lncRNA SNHG7 was highly expressed in gastric cancer tissues(P<0.01),miR-342-3 p was lowly expressed(P<0.01),and FOXM1 was highly expressed(P<0.01).Downregulation of lncRNA SNHG7 inhibited proliferation,invasion and EMT of MGC-803 cells.miR-342-3 p had a negative regulation and targeting relationship with FOXM1.Downregulation of miR-342-3 p promoted the proliferation,invasion and EMT

关 键 词：Lnc RNA SNHG7 miR-342-3p FOXM1 胃癌 增殖 EMT 

分 类 号：R735.2[医药卫生—肿瘤]