大肠腺瘤性息肉中医证型与P53基因、碱性成纤维细胞生长因子表达的相关性研究  

Study on Correlation Between Chinese Medicine Syndrome Types of Colorectal Adenomatous Polyps and Expression of P53 Gene and Basic Fibroblast Growth Factor

作　　者：张然[1] 陈正彦 杨坤 李素娟[1] ZHANG Ran;CHEN Zhengyan;YANG Kun;LI Sujuan

机构地区：[1]河南中医药大学第一附属医院脾胃肝胆科内镜中心,河南郑州450000

出　　处：《新中医》2022年第6期153-157,共5页New Chinese Medicine

基　　金：河南省中医药科学研究专项课题项目(2017JDZX002)。

摘　　要：目的:探讨大肠腺瘤性息肉不同中医证型与P53基因、碱性成纤维细胞生长因子(bFGF)表达的相关性。方法:选择大肠腺瘤性息肉患者100例作为研究对象,其中大肠湿热证组、脾胃虚弱证组、肝郁气滞证组、血瘀内停证组、寒邪内阻证组各20例设为研究组,所有患者均行电子结肠镜检查发现息肉并行息肉摘除术治疗;另选择正常人20例设为正常对照组。采用免疫组织化学法测定2组P53、b FGF表达阳性率并作比较;采用SPSS Pearson相关性分析软件对大肠腺瘤性息肉不同中医证型的P53基因、bFGF表达进行相关性分析。结果:研究组大肠腺瘤性息肉患者P53基因、bFGF阳性率均高于正常对照组,差异有统计学意义(P<0.05)。大肠腺瘤性息肉大肠湿热证组、脾胃虚弱证组P53基因、bFGF阳性率均高于肝郁气滞证组、寒邪内阻证组、血瘀内停证组,差异有统计学意义(P<0.05);大肠湿热证组P53基因、bFGF阳性率高于脾胃虚弱证组,差异有统计学意义(P<0.05);肝郁气滞证组、血瘀内停证组、寒邪内阻证组间P53基因、bFGF阳性率两两比较,差异无统计学意义(P>0.05)。大肠腺瘤性息肉患者大肠湿热证、脾胃虚弱证与P53基因、bFGF阳性率呈正相关性,差异有统计学意义(P<0.05);肝郁气滞证、血瘀内停证、寒邪内阻证与P53基因、b FGF阳性率无明显相关性(P>0.05)。结论:大肠腺瘤性息肉患者P53基因、bFGF阳性率表达较高,且在不同中医辨证分型下表达存在一定的相关性和统计学差异。Objective:To discuss the correlation between Chinese medicine syndrome types of colorectal adenomatous polyps and expression of P53 gene and basic fibroblast growth factor(bFGF). Methods:A total of 100 cases of patients with colorectal adenomatous polyps were selected as the study subjects. Patients in the large intestinal dampness-heat syndrome group,the spleen-stomach deficiency syndrome group,the liver depression and qi stagnation syndrome group,the internal stagnation of blood stasis syndrome group,and the internal obstruction of cold syndrome group were enrolled into the study group, with 20 patients per syndrome;all patients underwent electronic colonoscopy to locate polyp and treated with polypectomy. The control group included 20 normal people. The positive expression rates of P53 gene and bFBF were determined by immunohistochemistry and compared between the two groups. Correlation analysis of P53 gene and bFGF expression among different Chinese medicine syndrome types of colorectal adenomatous polyps was performed by SPSS Pearson correlation analysis software. Results:The positive rates of P53 and bFBF in patients with colorectal adenomatous polyps in the study group were higher than those in the control group,the difference being significant(P<0.05). The positive rates of P53 gene and bFBF in patients with colorectal adenomatous polyps in the large intestinal dampness-heat syndrome group and the spleen-stomach deficiency syndrome group were higher than those in the liver depression and qi stagnation syndrome group, the internal stagnation of blood stasis syndrome group, and the internal obstruction of cold syndrome group,the difference being significant(P<0.05). The positive rates of P53 gene and bFBF in the large intestinal dampnessheat syndrome group were higher than those in the spleen-stomach deficiency syndrome group, the difference being significant(P<0.05). When compared positive rates of P53 gene and bFBF among the liver depression and qi stagnation syndrome group, the internal stagnation of blo

关 键 词：大肠腺瘤性息肉 中医证型 P53基因 碱性成纤维细胞生长因子 相关性 

分 类 号：R735.34[医药卫生—肿瘤]