Fucoxanthin suppresses OxLDL-induced inflammation via activation of Nrf2 and inhibition of NF-κB signaling  

作　　者：Peramaiyan Rajendran Abdullah M AlZahrani 

机构地区：[1]Department of Biological Sciences,College of Science,King Faisal University,Al Ahsa 31982,Saudi Arabia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第5期207-215,共9页亚太热带生物医学杂志（英文版）

基　　金：Deanship of Scientific Research at King Faisal University Saudi Arabia,grant number 187006,funded this research.

摘　　要：Objective:To explore the impact of fucoxanthin on oxidized low-density lipoprotein(OxLDL)-induced stress and inflammation in human endothelial cells and its underlying mechanisms.Methods:HUVECs were treated with OxLDL and/or fucoxanthin for a range of time points and concentrations.We evaluated the effects of fucoxanthin on OxLDL-induced HUVECs using the MTT assay,reactive oxygen species accumulation assay,ELISA,RT-PCR,immunofluorescence,and Western blotting.Results:Fucoxanthin enhanced the cell viability in a dose dependent manner after OxLDL exposure.Furthermore,fucoxanthin pretreatment significantly decreased OxLDL-induced reactive oxygen species production and prevented the activation of the nuclear factor kappa-B pathway,which led to substantial suppression of pro-inflammatory gene expressions.OxLDL-induced upregulation of interleukin-6,intercellular adhesion molecule-1,vascular cell adhesion molecule-1,interleukin-1β,monocyte chemotactic protein-1,cyclooxygenase-1,and tumor necrosis factor-αwas significantly reduced by fucoxanthin.Conclusions:Fucoxanthin can inhibit OxLDL-induced vascular inflammation and oxidative stress in HUVECs by targeting Nrf2 signaling pathways.

关 键 词：Oxidized low-density lipoprotein FUCOXANTHIN Atherosclerosis INFLAMMATION Oxidative stress Cell viability HUVEC Nrf2 signaling pathway NF-ΚB 

分 类 号：R285[医药卫生—中药学]